Abstract
A strain of Rhodococcus kroppenstedtii was capable of synthesizing gold and silver nanoparticles (AuNPs and AgNPs). R. kroppenstedtii formed AuNPs in a cell associated manner that could be liberated after sonication. Extracellular AgNPs were produced when cell extracts (lysates obtained after incubation of cells with distilled water) were reacted with silver nitrate. For efficient synthesis of nanoparticles, different physicochemical parameters such as contents of chloroauric acid or silver nitrate, time, temperature and pH were optimized. The size, topology, dispersity and crystalline nature of the nanoparticles were ascertained by different analytical techniques. Six oligopeptides namely, Ala-Lys-Ala, Tyr-Glu, Ile-Leu-Leu, Leu-Gln-Ile, Gln-Glu-Lys and Pro-Trp-Lys were identified to mediate nanoparticle synthesis. The obtained AgNPs displayed antimicrobial and antibiofilm properties towards two primary aquaculture pathogens Vibrio harveyi and Pseudomonas aeruginosa. This study is novel as it highlights the isolation of R. kroppenstedtii from mangrove soils for the first time and details the significance of AgNPs derived from this isolate in controlling aquaculture pathogens as an effective alternative strategy.
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