Abstract
Nanolayered double hydroxide (LDH) is a type of anion layered inorganic compound whose bacteriostatic properties have recently garnered much attention. Vibrio parahaemolyticus is a marine pathogen that can lead to aquaculture diseases and substantial economic losses. Therefore, our study assessed the mechanisms by which Mg/Al-LDH prevents V. parahaemolyticus infection. Our results demonstrated that Mg/Al-LDH not only inhibited V. parahaemolyticus growth but also biofilm formation. Moreover, coupling Mg/Al-LDH with hydrogen peroxide and UV irradiation further inhibited the growth and biofilm formation of V. parahaemolyticus. Additionally, Mg/Al-LDH was found to adversely affect DNA and the gelling ability of chitosan. Furthermore, exposing V. parahaemolyticus to Mg/Al-LDH led to a 54.73% and 4.3% inhibition in the expression of the toxic genes tlh and trh, respectively. Mg/Al-LDH also improved the symptoms of V. parahaemolyticus infection in Penaeus vannamei, making this a promising candidate to prevent pathogenic bacteria infection in aquaculture.
Highlights
Nanolayered double hydroxide (LDH), known as anionic clay [1], is a type of anionic layered compound consisting of a positively charged metal hydroxide outlayer and a negative anion interlayer [2]
Our findings indicated that Mg/ Al-LDH prevented V. parahaemolyticus in Penaeus vannamei, highlighting the promising potential of Mg/AlLDH as an antibacterial agent to increase aquaculture yields
Mg/Al-LDH significantly inhibited V. parahaemolyticus growth at a 100 Mg/Al-LDH concentration (mg/L) concentration (Figure 1(a)), with OD values reaching only 90% compared to the control
Summary
Nanolayered double hydroxide (LDH), known as anionic clay [1], is a type of anionic layered compound consisting of a positively charged metal hydroxide outlayer and a negative anion interlayer [2]. 1[)M, wII1h-exMreIIMIx(IOII Hre)2p]rxe+s(eAnnt-s)Xt/rni·vyaHle2nOt metal ions, MII represents divalent metal ions, An- represents n-valent anions, and LDH represents the different structures that can be synthesized by changing the value of x [3, 4]. LDH with various unique properties can be synthesized through different combinations of divalent metal ions and trivalent metal ions, as well as changes in proportion [1, 6]. An LDH prepared by Moaty et al exhibited long-lasting antibacterial activity against Gramnegative bacteria (Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa) and Grampositive bacteria (Staphylococcus epidermidis, Staphylococcus aureus, Aspergillus, Streptococcus pyogenes, and Salmonella)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
