Nanolayer biofilm coated on magnetic nanoparticles by using a dielectric barrier dischargeglow plasma fluidized bed for immobilizing an antimicrobial peptide

Abstract

Using the monomer of acrylic acid and the novel technique of using a dielectric barrierdischarge glow plasma fluidized bed (GPFB), a nanolayer biofilm of polyacrylic acid (PAA)was uniformly coated on the surface of magnetic nickel nanoparticles (NPs). Transmissionelectron microscopy, Fourier transform infrared spectroscopy, and x-ray photoelectronspectroscopy, etc, were used to characterize the modified NPs. The thickness of the biofilmwas about 2 nm when the NPs were treated using the GPFB once, and the dischargingconditions affected the density of the carboxyl group obviously. The PAA acting as anadhesion layer was used to immobilize the antimicrobial peptide LL-37, to kill the bacteriaof Escherichia coli (E. coli), and the results indicated that the modified nickel NPsimmobilizing a certain concentration of LL-37 could kill the bacteria effectively.