Abstract
This study aimed to optimize extracellular glutathione production by a Saccharomyces cerevisiae engineered strain and to concentrate the extracellular glutathione by membrane separation processes, including ultrafiltration (UF) and nanofiltration (NF). Synthetic defined (SD) medium containing 20gL(-1) glucose was fermented for 48h; the fermentation liquid was passed through an UF membrane to remove macromolecules. Glutathione in this permeate was concentrated for 48h to 545.1±33.6mgL(-1) using the NF membrane; this was a significantly higher concentration than that obtained with yeast extract peptone dextrose (YPD) medium following 96h NF concentration (217.9±57.4mgL(-1)). This higher glutathione concentration results from lower cellular growth in SD medium (final OD600=6.9±0.1) than in YPD medium (final OD600=11.0±0.6) and thus higher production of extracellular glutathione (16.0±1.3 compared to 9.2±2.1mgL(-1) in YPD medium, respectively). Similar fermentation and membrane processing of sweet sorghum juice containing 20gL(-1) total sugars provided 240.3±60.6mgL(-1) glutathione. Increased extracellular production of glutathione by this engineered strain in SD medium and subsequent UF permeation and NF concentration in shortend time may help realize industrial recovery of extracellular glutathione.
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