N-Acetylation in healthy and diseased children

Abstract

Acetylation capacity was examined in three groups of Czech children by measuring the plasma and urine concentrations of sulphamethazine and its acetylated metabolite 6 h after an oral test dose of 20 mg/kg sulphamethazine. Amongst 82 healthy children aged 4-15 y there were 32 (39%) fast acetylators; there was no significant difference between the number of boys and girls, or between children over or less than 6 years of age. In 41 patients aged 3-15 y with phenylketonuria, the acetylation indices showed a significantly higher proportion of fast acetylators - 24 (58.5%) using plasma measurements and 29 (70.7%) using urine data. In them the ratio between slow and fast acetylators was inverted compared to normal children. The preponderance of fast acetylators was greater in boys than in girls and in children over 6 years of age. An increased acetylation capacity in patients with phenylketonuria was apparent even in individuals classified as slow acetylators, because in them the plasma concentration of the acetylated metabolite was higher than in control acetylators. Amongst 48 young patients (5-15 y) with insulin-dependent diabetes there were 19 (39.6%) fast and 29 (60.4%) slow acetylators, which corresponded well to the phenotype distribution in control children. This did not support the suggested association between the fast acetylator phenotype and Type I diabetes.