Abstract
[ 3H]-Ouabain binding to muscle preparations was utilized to estimate the number of Na +,K +-ATPase enzyme units in hindlimbs from 8 week old lean and obese mice. Specific [ 3H]-ouabain binding per mg particulate protein was 36% lower in obese mice; whereas, the affinity of the binding sites for ouabain was similar in obese and lean mice. Since obese mice had less muscle than lean mice, the number of Na +,K +-ATPase enzyme units in hindlimbs from obese mice was less than half the number observed in lean mice.
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