Abstract
The determination of intracellular elemental concentrations in cryo-sections of cardiac tissue is influenced by the presence of extracellular space (ECS). Intracellular elemental concentrations are determined by using a perfusion buffer with Na 4Lu(III)HDOTP as an extracellular marker, thus making it possible to correct for the ECS. Results show that when a regular perfusion buffer is replaced by a buffer containing 5 mM Na 4Lu(III)HDOTP, the contractility of an isolated perfused rat heart reduces but recovers completely within a few minutes. The high sensitivity of particle-induced X-ray emission (PIXE) for Lu and the possibility to add high concentrations of the Lu-buffer without significant physiological effects enables mapping of intracellular elemental concentrations in heart tissue.
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