Abstract
Shotgun proteomics based on liquid chromatography/mass spectrometry has been widely used to provide a large-scale view of the proteome, including protein expression, localization, modification, and protein-protein interaction. However, it is still challenging tasks to carry out membrane protein-targeted proteome analysis due to the hydrophobic nature of proteins with transmembrane domains. Here, we review current sample preparation techniques such as enrichment, solubilization, and digestion for membrane proteomics and focus on phase-transfer surfactant-based approaches recently developed by our group. We also discuss future developments for membrane proteome focused shotgun proteomics.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
