Abstract
Serum cortisol level of kidney transplant patients treated with prednisolone as an immunosuppressive agent was determined by a combination of rapid-flow fractionation (RFF) and high performance liquid chromatograpy (HPLC). A 0.5ml serum was extracted with ether using RFF technique prior to HPLC to obtain cortisol fraction. The ether extract was analyzed by HPLC with normal phase silica gel column and organic solvent system as a mobile phase solvent. Detection limit of this method was as low as 500pg/ml of serum.The cortisol peak was not disturbed by concomitant drugs such as azathioprine or cyclosporin A.
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