Abstract
A culture experiment was made to obtain biological information on the role of compounds in the mass production of microalga for four species, Tetraselmis tetrathele, Pavlova lutheri, Chaetoceros gracilis, and Navicula ramosissima. The compounds used were four kinds of carboxylic acids, citric acid, L-malic acid, fumaric acid, succinic acid, and their sodium salts. The culture of the microalgae was made using enriched seawater (PES, PESSi) with differentconcentrations of each compound.The growth of alga was accelerated by the addition of carboxylic acid with an optimal concentration: 100μg/ml of citric acid or succinic acid for T. tetrathele, 30μg/ml of citric acid or malic acid for P. lutheri, and 30μg/ml of citric acid, malic acid, or succinic acid for C. gracilis and N. ramosissima, respectively. The growth of alga was also accelerated by the addition of carboxylate with an optimal concentration: 100μg/ml of citrate for T. tetrathele, and 100μg/ml of citrate or malate for P. lutheri, C. gracilis, and N. ramosissima, respectively. Each one of the compounds was effective to promote the growth of these microalgae, although the effect for growth promotion may depend on the compounds added.
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