Abstract
Protein kinase A (PKA), protein kinase G (PKG) and protein kinase C (PKC) have crucial roles in signal transduction of smooth muscle cell. However, little is known about a shift of gene expression when PKA, PKG and PKC are activated in the smooth muscle cells. And the exact pathways of signal transduction from activation of PKA, PKG and PKC to regulation of gene expression in the smooth muscle cells are not well understood. Recently, RNA fingerprinting by arbitrarily primed PCR (RAP-PCR) has been developed as a method to identify and characterize alter gene expression in eukaryotic cells. Fingerprinting of RNA populations was achieved using anarbitrarily selected primer at low stringency for first and second strand cDNA synthesis. In this report, we have characterized forskolin, sodium nitroprusside (SNP) and 12-O-tetradecanoylphorbol-13-acetate (TPA) induced alterations of gene expression in cultured rat smooth muscle cells using the RAP-PCR method. After incubation with forskolin, SNP and TPA in cultured rat aortic smooth muscle cells, total RNA was isolated and converted to cDNA using an arbitrary primer. Each three agents induced a variety of differential gene expression. These results suggest that several gene expressions were selectively dependent on each intracellular signaling in cultured rat aortic smooth muscle cells.
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