Abstract
N2O is a typical greenhouse gas, and contributes severely to global warming. The process for biological nitrogen removal from wastewater is considered as an important source of N2O emission. The present study investigated the characteristics and main source of N2O in a SBR system, with an anoxic-aerobic (A/O) running mode. The results showed that N2O emission occurred mainly during the aerobic phase in the A/O SBR system. The maximum N2O emission rate reached 2.02 μg/(min·g), and the cumulative N2O emission during the aerobic phase was 8.2 mg, then the nitrite accumulation concentrate was observed to get the highest value of 7.5 mg/L after 120-minite operation. On the basis of bacterial community analysis, the bacteria in the dominant DGGE bands were identified as Flavobacteria, some of which was found to be aerobic denitrifying bacteria. It was inferred that the accumulation of nitrite might inhibit the activity of nitrous oxide reductase (Nos) from aerobic denitrifying bacteria and lead to N2O emission. The accumulation of nitrite should be avoided or reduced in real biological wastewater treatment process.
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