Abstract
BackgroundA considerable number of muscle development-related genes were differentially expressed in the early stage of avian adipocyte differentiation. However, the functions of them in adipocyte differentiation remain largely known. In this study, the myoblast determination protein 1 (MYOD1) was selected as a representative of muscle development. We investigated its expression, function, and regulation in avian adipocyte differentiation.ResultsThe expression of MYOD1 decreased significantly in the early stage of avian adipocyte differentiation. CRISPR/Cas9-mediated deletion of MYOD1 induced adipocyte differentiation, whereas over-expression of MYOD1 inhibited adipogenesis. The mRNA-seq data showed that MYOD1 could perturb the lipid biosynthetic process during differentiation. Our results showed that MYOD1 directly up-regulates the miR-206 expression by binding the upstream 1200 bp region of miR-206. Then, over-expression of miR-206 can inhibit the adipogenesis. Furthermore, MYOD1 affected the expression of endogenous miR-206 and its target gene Kruppel-like factor 4 (KLF4), which is an important activator of adipogenesis. Accordingly, the inhibition of miR-206 or over-expression of KLF4 could counteract the inhibitory effect of MYOD1 on adipocyte differentiation.ConclusionsOur results establish that MYOD1 inhibits adipocyte differentiation by up-regulating miR-206 to suppress the KLF4 expression. These findings identify a novel function of MYOD1 in adipocyte differentiation, suggesting a potential role in body-fat distribution regulation.
Highlights
A considerable number of muscle development-related genes were differentially expressed in the early stage of avian adipocyte differentiation
These findings identify a novel function of myoblast determination protein 1 (MYOD1) in adipocyte differentiation, suggesting a potential role in body-fat distribution regulation
These results indicate that MYOD1 is involved in the avian adipocyte differentiation process
Summary
A considerable number of muscle development-related genes were differentially expressed in the early stage of avian adipocyte differentiation. The functions of them in adipocyte differentiation remain largely known. The myoblast determination protein 1 (MYOD1) was selected as a representative of muscle development. Function, and regulation in avian adipocyte differentiation. Adipogenesis is driven by an Several studies and observations showed that fat deposits in the muscles will cause the loss of muscle quality and are more likely to induce metabolic diseases in various animals [5,6,7,8]. It has been shown that peroxisome proliferator-activated receptor γ (PPARγ) and myoblast determination protein 1 (MYOD1) are the master regulators of adipogenesis and myogenesis, respectively [17, 18]. The functional contributions of muscle development genes to adipocyte differentiation remain largely unexplored
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
