Myoblast Communication and Antibacterial Potential of Poly(lactic acid) Nanoscaffolds: Advancing Musculoskeletal Tissue Engineering Implants

Abstract

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Myogenic bioengineering has emerged as a promising field aimed at repairing or regenerating damaged muscle tissues triggered by trauma, diseases, or congenital defects. Poly(lactic acid) (PLA) nanofibrous scaffolding has appealed to progressive attraction as an innovative support for prospective therapy in renewal/or repair medicine. Herein, high aspect ratio PLA nanofibers were fabricated using electrospinning and characterized by means of various sophisticated techniques such as XRD, SEM, TGA and FTIR spectroscopy. There is no information regarding the interaction of muscle cells with PLA. Thus, muscle precursor cells were exposed to various doses of electrospun PLA nanoscaffolds to test proliferation and in vitro biocompatibility. Cell viability was assessed by CCK-8 at standard time periods. The structure of treated myoblasts was scanned by a phase contrast microscope and to check the growing profile Bio-SEM was used. Furthermore, antimicrobial activity was explored against Gram-positive Staphylococcus aureus. The findings specify that PLA nanoscaffolds exhibit mild antimicrobial efficiency. Besides, the myoblasts demonstrated proliferative behavior on nanoscaffolds. Our investigation highlights that PLA nanofibers hold significant promise for developing biomimetic cellular scaffolds. Moreover, the PLA nanoscaffolds developed in this study show great potential for tissue engineering, particularly in muscle regeneration. Its high biocompatibility and ability to support myoblast attachment, proliferation and differentiation make it a promising biomaterial implant for regenerative medicine.