Abstract
Recombinant protein technology has emerged as an excellent option for vaccine development. However, prior to our study, the immune induction ability of recombinant Mycoplasma suis alpha-enolase (rMseno) in animals remained unclear. The purpose of this study was to develop a rMseno protein subunit vaccine and to determine its ability to elicit an immunological response. To accomplish this, we cloned the gene into pET-15b, expressed it in BL21 cells, and purified it. Following the establishment of immunity, the immunogenicity and potential for protection of rMseno were evaluated in mice and piglets. The results demonstrate that anti-M. suis serum recognized the pure rMseno protein in both mice and piglets as evidenced by high levels of specific anti-rMseno antibodies, significantly increased levels of IFN-γ and IL-4 cytokines, and significantly increased T lymphocyte proliferation index. Piglets also had significantly increased levels of specific IgG1, IgG2a, CD4+, and CD8+ cells. The rMseno findings demonstrated a robust immunological response in mice and piglets, affording partial clinical protective efficacy in piglets.
Highlights
The M. suis alpha-enolase gene was successfully amplified at a length of 1632 bp, an increase of 9 bp over the initial length before optimization
Coli-Mseno stimulated groups A and. These results demonstrated that both recombinant Mycoplasma suis alpha-enolase (rMseno) and coli-Mseno stimulated the production of a strong cellular immune response
These results demonstrated that both rMseno and E. coli-Mseno stimulated the production of a strong cellular immune response
Summary
Mycoplasma suis (M. suis) is a hemotrophic mycoplasma that has not been successfully cultured in vitro to date [1,2,3,4,5,6]. In pigs, it binds to the surfaces of red blood cells (RBCs), causing erythrocyte malformation and chronic or acute anemia [6,7,8,9,10]. In acute cases, infected pigs showed high fever, hemolytic anemia, and death in clinical trials. M. suis infections have been reported worldwide, including in China, and have resulted in significant economic losses in the swine industry [14,15,16,17]
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