Mycobacterium Tuberculosis Protocols

Abstract

Plasmid Vectors Nicola Casali and Sabine Ehrt Isolation of DNA from Mycobacterium tuberculosis Paul D. van Helden, Thomas C. Victor, Robin M. Warren, and Eileen G. van Helden Extraction of RNA from Intracellular Mycobacterium tuberculosis: Methods, Considerations, and Applications Irene M. Monahan, Joseph A. Mangan, and Philip D. Butcher Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages Stoyan S. Bardarov, Svetoslav S. Bardarov, Jr., and William R. Jacobs, Jr Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB Mary Jackson, Luis Reinaldo Camacho, Brigitte Gicquel, and Christophe Guilhot Gene Replacement Using Pretreated DNA Bhavna G. Gordhan and Tanya Parish Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL+ as a Dominant Negative Selectable Marker Peter Sander, Burkhard Springer, and Erik C. Boettger Transcription Start-Site Mapping Farahnaz Movahedzadeh, Jorge A. Gonzalez-Y-Merchand, and Robert A. Cox Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells Rory P. Cooney, Natalie J. Garton, and Michael R. Barer Phage Replication Technology for Diagnosis and Drug Susceptibility Testing Ruth McNerney Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy Thomas C. Victor and Paul D. van Helden Restriction Fragment Length Polymorphism Typing of Mycobacteria Dick van Soolingen, Petra E. W. de Haas, and Kristin Kremer Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis Ida Rosenkrands and Peter Andersen Analysis of the Capsule of Mycobacterium tuberculosis Mamadou Daffe and Marie-Antoinette Laneelle Analysis of the Lipids of Mycobacterium tuberculosis Richard A. Slaydenand Clifton E. Barry, 3rd In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis Lawrence G. Wayne Macrophage Virulence Assays Pauline T. Lukey and Elizabeth U. Hooker Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation Wandy Beatty and David G. Russell Real-Time PCR Using Molecular Beacons: A New Tool to Identify Point Mutations and to Analyze Gene Expression in Mycobacterium tuberculosis Riccardo Manganelli, Sanjay Tyagi, and Issar Smith Electronic Access to Mycobacterium tuberculosis Sequence Data Julian Parkhill Proteomics Joanna C. Betts and Marjorie A. Smith Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays Michael Wilson, Martin Voskuil, Dirk Schnappinger, and Gary K. Schoolnik Storage of Mycobacterial Strains Kristin Kremer, Tridia van der Laan, and Dick van Soolingen Safety in the Laboratory Heidi Alderton and Debbie Smith