Abstract
Extracellular polysaccharides (EPS) produced by marine microalgae have the potential to be used as antioxidants, antiviral agents, immunomodulators, and anti-inflammatory agents. Although the marine microalga Crypthecodinium cohnii releases EPS during the process of docosahexaenoic acid (DHA) production, the yield of EPS remains relatively low. To improve the EPS production, a novel mutagenesis of C. cohnii was conducted by atmospheric and room temperature plasma (ARTP). Of the 12 mutants obtained, 10 mutants exhibited significantly enhanced EPS yield on biomass as compared with the wild type strain. Among them, mutant M7 was the best as it could produce an EPS volumetric yield of 1.02 g/L, EPS yield on biomass of 0.39 g/g and EPS yield on glucose of 94 mg/g, which were 33.85%, 85.35% and 57.17% higher than that of the wild type strain, respectively. Results of the present study indicated that mutagenesis of the marine microalga C. cohnii by ARTP was highly effective leading to the high-yield production of EPS.
Highlights
Polysaccharides are polymeric carbohydrate molecules consisting of long chains of monosaccharide units
To find the best sampling time, C. cohnii that grew after 24, 36, and 72 h were sampled to perform the ARTP treatment, which were corresponding to the beginning of log phase, the end of log phase, and the end of stationary phase, respectively
The plasma of ARTP system may lead to a lethal, cell permeability change or DNA damage of C. cohnii at the end of exponential phase
Summary
Polysaccharides are polymeric carbohydrate molecules consisting of long chains of monosaccharide units. As a major class of biological macromolecules, they play important roles in a vast number of biochemical activities [1]. With the development of glycomics, the significant contribution of polysaccharides in human health has received increasing attention in recent years. There are a growing number of studies demonstrating the bio-modulatory effects of polysaccharides, e.g., anti-oxidative, anti-inflammatory, anti-adhesive, anti-coagulant, anti-cancer, anti-viral, and immunomodulatory properties [2,3,4]. The production of polysaccharides from marine microalgae is practically advantageous because the process is controlled and the harvesting does not depend on the climate or season [11]
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