Abstract
The mutagenic effect of sodiurn azide was studied under several treatment conditions in dry and pre-soaked seeds of barley. In dry seeds, the germination rate and seedling growth were reduced in proportion to the increase in concentration of sodium azide. The highest limit of chlorophyll mutation frequency was about 3xlO-1 per M1 spike and lxl0-1 per M2 seedling. The percentages of various types of chlorophyll mutation under all these treatment conditions were 47.8, 36.6 and 15.6 for albina, viridis and others (xantha, striata etc.) respectively. In presoaked seeds, the growth inhibiting effects of sodium azide did not increase with the prolongation of pre-soaking time. The highest value of chlorophyll mutation frequency was observed when sodium azide was applied at the S-phase in the first postdormant mitotic cycle. The frequency of visible mutants followed the same pattern. From these results, it is concluded that sodium azide is an effective mutagen and also that this agent is most effective in inducing mutation when applied at the S-phase.
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