Muscarinic cholinergic receptor subtypes in rat sublingual glands

Abstract

Mucin glycoprotein secretion by rat sublingual glands is regulated primarily by muscarinic cholinergic receptors. Studies were conducted to identify muscarinic receptor subtypes in whole glands as well as in isolated acinar structures. In radioligand binding studies, we used subtype-selective antagonists in competition studies to initially determine receptor subtype heterogeneity. In membranes from whole glands, both pirenzepine and methoctramine displayed two affinity sites (M1 and M3) of nearly equal proportions. In contrast, acinar membranes contained a 1:2 and 2:1 ratio of M1 to M3 sites for pirenzepine and methoctramine, respectively. In all cases, p-fluoro-hexahydro-siladifenidol and 4-diphenylacetoxy-N-methylpiperidine each bound to a single class of binding sites. Northern analysis using oligonucleotide probes specific for the 5' ends of the translated regions of m1 through m5 receptors detected only m1 and m3 subtypes in poly(A)+ RNA from whole glands. We also used antisera specific for each receptor subtype to immunoprecipitate solubilized receptors from membrane preparations. Only m1 (51.7 and 64.9%) and m3 (48.3 and 34.7%) subtypes were found consistently in membranes from whole sublingual glands and isolated acini, respectively. Studies with other exocrine glands generally described the predominance of m3 receptors, and m1 receptors, if present, were presumably associated with contaminating neural structures. Our results therefore demonstrate that mucous acini from rat sublingual glands contain abundant amounts of both m1 and m3 receptors.