Abstract
Isotope analysis has proved to be an extremely important tool in the traceability process; however, statistical analyses of the results show discrepancies, as the data depend on and originate from several chemical elements such as carbon, hydrogen, oxygen, nitrogen and sulfur (CHONS). In order to establish the proper analysis of traceability data for birds using the stable isotope technique and evaluate the need for a combined analysis of the variables, data for carbon-13 and nitrogen-15 were used from eggs (albumen + yolk) of laying hens and the pectoral muscle of broilers, which were subjected to univariate statistical analysis (ANOVA and complemented with Tukey’s test) and multivariate statistical analysis (MANOVA and Discriminant Analysis). The data were analyzed using Minitab 16 software, and the results, corroborated in the theory, confirm the need for multivariate analysis, showing also that discriminant analysis clarifies questions from the results of the other analysis methods compared in this study.
Highlights
In animal traceability, the isotopic composition of carbon in tissues serves as a natural tracer of the different diets, each with distinct isotopic signatures
The data were explored by multivariate analysis of variance (MANOVA) multivariate analyses, evaluating the comparison between the standard (0% inclusion) and the other treatments, represented by the twodimensional graph, as well as discriminant analysis
A significant difference was evident between treatments, through univariate analysis of variance (ANOVA) (Table 1) for variable carbon-13 in data on eggs
Summary
The isotopic composition of carbon in tissues serves as a natural tracer of the different diets, each with distinct isotopic signatures. Some authors have evaluated the stable isotopes of carbon and nitrogen independently (univariate analysis). Statistical analysis of the results for carbon and nitrogen was performed independently, that is, for the whole egg and its components (yolk and albumen) and for each variable (carbon and nitrogen). The author observed that the results for the variables carbon and nitrogen were different within the same sample; it was concluded that the analysis of stable nitrogen isotopes in egg components is a potentially useful technique to determine the diet to which the birds were submitted, serving as an important authentication tool in the egg industry
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
