Abstract
The enzymatic saccharification of sugarcane bagasse was significantly increased by alkali pretreatment under mild conditions. The effectiveness of different concentrations of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of lignocellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% celluloses, 25.63% hemicelluloses, and 30.36% lignin. After that, sugarcane bagasse was pretreated with 5 and 10% of H2SO4 and NaOH at 121°C for 60 min. FTIR, XRD, and SEM analyses also showed significant molecular and surface structure changes of the sugarcane bagasse with 10% NaOH. Maximum saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75, 301.25, and 276.6 mg/g from 10% H2SO4, 5% NaOH, and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1, and 20.6 times higher than the that of the control. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 (MK443365) and Bacillus paramycoides T4 (MN370035) were used to hydrolyze the pretreated bagasse, and the optimal condition was determined to be 30 h of the enzymatic reaction with the 3:1 ratio of enzymes under the temperature of 55°C, pH 5.0, and substrate concentration of 3%, leading to celluloses and hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.
Highlights
Sugarcane is one of the most popular crops in India with more than 5 million hectares of land under cultivation
Indigenous sugarcane bagasse was applied for chemical composition analysis and found that sugarcane bagasse contains cellulose (39.52%), hemicellulose (25.63%), total lignin (30.36%), ash (1.44%), and extractives (2.90%) (Table 1)
In contrast to our result, Moretti et al (2014) observed that 46.9% cellulose, 16.3% hemicellulose, 27.1% lignin, and 2.0% ash were found in sugarcane bagasse
Summary
Sugarcane is one of the most popular crops in India with more than 5 million hectares of land under cultivation. The average yield os sugarcan is more than 75000 kg/hectare with the total production exeeding 360 million tonnes and 110 million ton of bagasse, a solid waste resulting from juice extraction in 2019. Uttar Pradesh has the largest cultivable land of around 21lakh hectares with an annual output of 133.3 million tonnes. Uttar Pradesh stands proudly at the top of the list, second and third largest states are Maharashtra and Karnataka. India hold the second rank in the world after Brazil as far as sugarcane production is concerned. The generation of bioethanol can decrease the import of petroleum, and thereby increase the autonomy of energy growth in a country (energy security), such as the United States and Brazil (Demirbas 2011)
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