Abstract
Application of the short tandem repeats (STRs) loci located in the X chromosome has become an efficient tool in forensic kinship testing. The aim of this study is to develop a new multiplex that analysis of 19 X-STRs loci (DXS9895, GATA144D04, DXS10077, DXS10078, DXS10161, DXS10160, DXS981, DXS6800, DXS6803, DXS9898, DXS6801, DXS6799, DXS6797, DXS7133, DXS6804, GATA172D05, DXS8377, DXS10146, and DXS10147) simultaneously in a single reaction and to evaluate the allele and haplotype diversity of this multiplex system in the Chinese Han population. In addition, the sensitivity test showed a complete genotyping profile with DNA as low as 0.25ng. The results indicate that the new 19 X-STRs multiplex system may provide high polymorphism information and could be used as a supplementary tool for kinship testing as well as X chromosome genetic mapping studies in China.
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