Abstract
Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exact multiplexing. Lateral flow immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis, thanks to its simplicity, rapidity, and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing, and is therefore a possible answer to the pressing demand of multiplexing point-of-need analysis. This review presents an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent, and colored labels.
Highlights
A lateral flow device (LFD) is a particular type of biosensor, in which the recognition layer is fabricated onto the surface of a porous membrane
But not necessarily, the recognition elements are specific antibodies, and the biosensor relies on immunoassay principles, giving rise to the so-called ‘lateral flow immunoassay’ (LFIA), called the immunochromatographic test
The NALFIA technique employs the architecture of LFA devices and similar signal reporters, while the recognition element is represented by a DNA or RNA probe
Summary
A lateral flow device (LFD) is a particular type of biosensor, in which the recognition layer is fabricated onto the surface of a porous membrane. A LFD includes at least twoby reaction the distance between these zones obviously leads to increased consumption of sample and membrane sites, one that responds to the compound to be detected (called the test line), and a second reaction materials, as well as as increased assay timesto[21,22]. The spatial separation of multiple detection sites requires the minimum manipulation of the assay architecture (Figure 2a) and is the far most popular way to achieve multiplexing in LFIA [6,7,8,9,10,11,12,13,14,15,16,17,18,19,20].
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