Abstract
Human interleukin-1β (rhuIL-1β), obtained by DNA recombinant technology, was radiolabelled. Its isoelectric properties were determined by various analytical techniques such as high-voltage ultrathin layer isoelectric focusing (IEF) and chromatofocusing. The rhuIL- 1β molecule had a molecular mass of 18 kDa, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. When examined by IEF on a polyacrylamide gel of 1 mm thickness in the pH range of 3.5–9.5, it was resolved into two broad bands appearing in the pH range of 6.2–5.8 and 5.5–5.2. Each of the two bands was further resolved into multiple bands when electrofocused on (i) a thinner gel of 0.5 mm thickness and (ii) a narrower pH range of 5–8. Upon chromatofocusing in a liquid column, it was possible to isolate various charged components of rhuIL-1β. However, all these components reacted to the antiserum to rhuIL-1β and displayed a molecular mass of 18 kDa suggesting the charge heterogeneity of rhuIL-1β.
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