Multiple Biotherapy Effects of Salidroside on Gastric Carcinoma

Abstract

To verify the anti-tumor effect of salidrosideon gastric cancer, BALB/C mice grafted with a mouse gastric adenocarcinoma cell line MFC was used as the experimental model. The mice were divided into four groups, one control and the other three different drug administration experimental groups. Animals in the three experimental groups received Shenfoweikang herb over a 60-day period starting at the first day after grafting. Animals received saline as controls. All the mice were sacrificed at 61 days after being grafted. The anti-tumor effect was assessed by three ways: (1) tumor size was periodically measured during the life of the animals and tumor weight was determined by an electron balance immediately after the animals killed. (2) Apoptotic indices (AI) were examined by the TUNEL method and flow cytometric analysis. (3) The expression of VEGF, STAT3 and DEC1 in tumor tissues was examined by immunostaining and analyzed by Image J analysis system. Compared with controls, tumor growth (size and weight) was significantly inhibited with salidroside treatment (P<0.05). AI in gastric cancergrafted mice was significantly increased in the salidrosidetreatment group compared with the controls. The expressions of VEGF, STAT3 and DEC1 in tumor tissues were down-regulated after treated with salidroside. The anti-tumor effect of gastric cancer cell growth in vivo by salidroside is related with the induction of the cell apoptosis and down-regulation of VEGF, STAT3 and DEC1 signal transduction system.