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Abstract
Plant cell culture has multiple applications in biotechnology and horticulture, from plant propagation to the production of high-value biomolecules. However, the interplay between cellular diversity and ambient conditions influences the metabolism of cultured tissues; understanding these factors in detail will inform efforts to optimize culture conditions. This study presents multiomics datasets from callus cultures of tobacco (Nicotiana tabacum), rice (Oryza sativa), and two bamboo species (Phyllostachys nigra and P. bambusoides). Over four weeks, calli were cultured under continuous moisture without airflow or gradually reduced ambient moisture with airflow. For each sample, gene expression was profiled with high-throughput RNA sequencing, 442 metabolites were measured using liquid chromatography (LC) with triple-quadrupole mass spectrometry (LC–QqQMS), and 31 phytohormones were quantified using ultra-performance LC coupled with a tandem quadrupole mass spectrometer equipped with an electrospray interface (UPLC-ESI-qMS/MS) and ultra-high-performance LC–orbitrap MS (UHPLC-Orbitrap MS). These datasets highlight the impact of airflow on callus cultures, revealing differences between and within species, and provide a comprehensive resource to explore the physiology of callus growth.
Highlights
Background & SummaryPlant cell cultures, historically used for conservation and molecular breeding, are seen as potential cell factories for sustainable plant-based production of high-value biomaterials including pharmaceuticals and cosmetics, facilitated by genetic engineering and advanced by synthetic biology[1–3]
Plant callus cultures derived from various plant species exhibit remarkable diversity in their metabolic signatures[4], as well as in their capacities for proliferation, regeneration, and differentiation[5]
Such diversity in callus cultures is often observed between plant species and even within the same species, influenced by various factors including the origin of the tissues[6], plant genotype[7], and the ambient conditions[8]
Summary
Historically used for conservation and molecular breeding, are seen as potential cell factories for sustainable plant-based production of high-value biomaterials including pharmaceuticals and cosmetics, facilitated by genetic engineering and advanced by synthetic biology[1–3]. 31 phytohormones were quantified using both ultra-performance LC (UPLC) coupled with a tandem quadrupole mass spectrometer (qMS/MS) equipped with an electrospray interface (ESI) (UPLC-ESI-qMS/MS) and ultra-high-performance LC–orbitrap high-resolution MS (UHPLC-Orbitrap MS), providing comprehensive profiles of these phytohormones. This multiomics dataset, the first to systematically capture data across diverse plant species’ callus cultures, provides foundational insights into the cellular states and responses of calli, enhancing our understanding of plant cellular physiology and supporting future efforts in metabolic engineering for efficient plant-based material production
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