Abstract
Focus on the problem of multi-component in-situ on-line monitoring in fermentation, in this work, we proposed an on-line in-situ multi-channel detection flow path control system based on a novel enzyme electrode prepared from Prussian Blue (PB) nanomaterials. To match the detection behavior of this electrode, an innovative design of a multi-channel pump-valve synergistic flow path control system can effectively reduce cross-contamination and improve system reliability. According to the flow path detection logic requirements, software program modules, such as precise positioning and micro-injection of pipette needle, and calibration detection during the multi-component detection process, have been developed. Precise positioning and micro-injection of pipette needle were realized by the trapezoidal acceleration and deceleration algorithm combined with a subdivision drive circuit. For the continuous change glucose concentration in fermentation, an adaptive sampling strategy of the injection volume and calibration detection module were proposed to realize multi-component, wide-range from 1g/L to 180g/L, fast and accurate detection, which presenting the as-prepared in-situ on-line system was proved to exhibits a high accuracy with a low detection error (<2%) within the linear range.
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