Abstract
The multi-functional micelles poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide-co-10 undecanoic acid)/CM-Dextran Fe3O4 (PNDU/CM-Dex Fe3O4) were poly (NIPAAm-co-DMAAm-co-UA) (PNDU) grafting hydrophilic CM-Dextran Fe3O4 which possess pH-dependent temperature response and magnetic response. In this research, anti-inflammation drug Hesperetin was encapsulated by micelles using membrane dialysis method to obtain the different ratio of Hesperetin-embedded P5DF10, P10DF10, and P20DF10. These micelles were characterized by Fourier transform infrared spectroscopy, 1H-NMR, thermogravimetric analyzer, and superconducting quantum interference device magnetometer. The morphology and particle size of micelles was observed by transmission electron microscopy and dynamic light scattering. The low critical solution temperature of the P10DF10 micelles is in pH 6.6 at about 37.76°C and in pH 7.4 at about 41.70°C. The biocompatibility of micelles was confirmed by cytotoxicity study. Inflammatory inhibition of hesperetin-embedded P10DF10 micelles also studied through RAW264.7. Hesperetin-embed P10DF10 micelles suppressed LPS-induced inflammatory response. Via immunofluorescence cell staining demonstrate that Hesperetin-embed P10DF10 micelles inhibited the activation of NF-κB p60 and markedly attenuated in a drug dose-dependent manner. At a concentration of 1,000 ug/ml, an inflammatory rate can be reduced to 36.9%. Based on these results, the hesperetin-embed P10DF10 micelles had successfully synthesized and enable to carry and release the anti-inflammatory drugs, which instrumental for biomedical therapy and applications.
Highlights
Inflammation is the common response to injured vascular living tissue
This study was based on the pH and temperature response polymer of poly(NIPAAm-co-DMAAm-co-undecenoic acids (UA))-COOCH3 and it was modified to poly(NIPAAm-co-DMAAm-co-UA)NHNH2; subsequently. grafting CM-Dextran/Fe3O4, led to the development of the multi-functional drug carrier micelles of poly(NIPAAM-co-DMAAm-co-UA)/carboxymethyl dextran (CM-Dex)-Fe3O4
This study regulated the composition of CM-Dextran/Fe3O4 and PNDU-NH2 to optimize low critical solution temperature (LCST) finding that it was suitable for the lesion area at a slightly higher temperature than normal tissues; it responded to the environments of normal tissue and lesions, which confirmed that the micelles had pH and temperature response behaviors
Summary
Inflammation is the common response to injured vascular living tissue. Microbial infections, inappropriate immunological responses or other issues may cause inflammation. The purpose of inflammation is to eliminate the microorganisms, enclose the injury, inactivate toxin so that the tissue or organ can be repaired Some inflammation processes can be an insidious effect on the noxious sensitive reaction in progressive organ damage with the inflammatory mediators especially occur in chronic inflammation (Shin et al, 2008). It is widely accepted that inflammation plays an important role in atherosclerotic plaque formation (Ross, 1999). Some approaches provided to overcome inflammation issue. Pan et al (2015) presented a useful strategy for the release of anti-inflammatory drug by loading enzyme-sensitive polymeric prodrug in the scaffold Some approaches provided to overcome inflammation issue. Pan et al (2015) presented a useful strategy for the release of anti-inflammatory drug by loading enzyme-sensitive polymeric prodrug in the scaffold
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
