Abstract
Mulinum crassifolium Phil. (Apiaceae) is an endemic shrub from Chile commonly used as infusion in traditional medicine to treat diabetes, bronchial and intestinal disorders and stomach ailments, including ulcers. From the EtOAc extract of this plant, the new mulinane-type diterpenoids 3 and 5 were isolated along with three known diterpenoids. The gastroprotective effect of the infusion of the plant was assayed to support the traditional use and a fast HPLC analysis using high resolution techniques was performed to identify the bioactive constituents. The EtOAc extract and the edible infusion showed gastroprotective effect at 100 mg/kg in the HCl/EtOH induced gastric ulcer model in mice, reducing lesions by 33% and 74%, respectively. Finally, a metabolomic profiling based on UHPLC-ESI-MS/HRMS of the edible infusion was performed and thirty-five compounds were tentatively identified including quercetin, caffeic acid, apigenine glucoside, p-coumaric acid, chlorogenic acids, and caffeoylquinic acids, which have been associated previously with gastroprotective and antiulcer properties. This scientific evidence can support the contribution of polyphenols in the gastroprotective activity of the edible infusion of this plant, and can validate at least in part, its ethnopharmacological use.
Highlights
Mulinum crassifolium (Apiaceae) is an endemic shrub confined to the north of Chile and commonly known as “chuquican” or “sucurco”
As part of our studies on mulinane-type diterpenoids from Apiaceae, we describe in this paper the isolation and structural elucidation of two new diterpenoids from M. crassifolium
We isolated two new mulinane-type diterpenoids from the ethyl-acetate extract from M. crassifolium using a combination of chromatographic techniques and identification by NMR
Summary
Mulinum crassifolium (Apiaceae) is an endemic shrub confined to the north of Chile and commonly known as “chuquican” or “sucurco”. Previous chemical studies in this genus have informed the presence of coumarins, aromatic acids, monoterpenes and mulinane-type diterpenoids so far [2,3,4,5,6,7]. In the case of M. crassifolium, some diterpenoids have been. Liquid chromatography coupled to mass spectrometry is considered a powerful method of analysis in environmental, food, and antidoping tests, very important in forensic laboratories as well as in applied research. Analytical challenges and developments in performance have been found in ion sources, especially those using atmospheric-to-vacuum interfaces such as atmospheric pressure chemical ionization (APCI), or the most common electrospray (ESI) and on the increasing use of high-resolution mass spectrometry (HR-MS) instead of low-resolution mass spectra
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