Abstract

ABSTRACT A new azimuthally stable polarimetric method for processing of microscopic images of optically anisotropic structures of different biological layers histological sections is proposed. A new model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity is proposed. The matrix element M 44 has been chosen as the main information parameter, which value is independent of rotation angle of both sample and probing beam polarization plane. Keywords: polarimetry, blood plasma, laser image, filtering. 1. INTRODUCTION Development of computational methodologies for processing of microscopic images is a new health-physical method. It is laser polarimetry of biological tissues histological sections [1 - 3]. It is based on measurement of the coordinate distributions (polarization maps) in the plane of polarization states of histological sections microscopic images of biological tissues. This method allows a new, inaccessible to the histological and mathematical methods of analysis, information on the optical anisotropy (linear and circular birefringence) multiscale structural elements of different biological objects. At the same time, a complex analysis of the polarization maps of a tissue specimen is azimuthally dependent to the probing beam polarization plane and sample rotation angle. This makes it difficult to use this method in comparative research groups histological sections with different pathologies. Thus, further progress of laser polarimetry may be connected with the development of azimuth ally stable methods of direct measurement of the parameters of linear and circular birefringence. The solution of this task is using of Mueller-matrix cartography with so-called rotational invariants. It has been shown in the works [4 - 6] that azimuthally stable, independent of the sample rotation angle (

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