MP86-19 EFFECT OF SLEEP DEPRIVATION ON HORMONAL AXIS AND ERECTILE FUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology I1 Apr 2016MP86-19 EFFECT OF SLEEP DEPRIVATION ON HORMONAL AXIS AND ERECTILE FUNCTION Dongsup Lee, Seung-ju Lee, and Dong-wan Sohn Dongsup LeeDongsup Lee More articles by this author , Seung-ju LeeSeung-ju Lee More articles by this author , and Dong-wan SohnDong-wan Sohn More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2327AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Testosterone contributes signal pathway of penile erection and it is mainly secreted during sleep. However, there is no consensus that which level of the hormone axis will be mainly affected to sleep deprivation (SD). So, we investigated whether 1) SD could alter the hormonal axis or not, 2) which level of the axis could be the major target of SD and 3) subsequently whether SD could decrease erectile function or not. METHODS 32 Wistar rats (16 weeks old) were divided into 2 groups. Using automated sleep deprivation system (Pinnacle technology inc. USA), sleep deprivation for 48~56 hours was done in experimental group. Then, cavernous nerve on posterolateral aspect of the prostate was stimulated with electrode. Intracavernosal pressure was checked during carotid arterial pressure monitoring. Blood sample was collected by puncturing of left cardiac ventricle. After sacrifice of a rat, brain tissue was extracted for measuring Kisspeptin mRNA, and fluorescence stains were done with testis (P450scc stain, stAR stain) and penile tissue (nNOS, eNOS). RESULTS Kisspeptin mRNA, gonadotropin releasing hormone, follicular stimulating hormone were not different between two groups. Luteinizing hormone and testosterone were significantly different between two groups (a_image). Steroidogenesis in the leydig cell was more prominent in control group than in experimental group. Positive area of cavernous nerve with nNOS staining and that of penile smooth muscle with eNOS staining were decreased in SD group. Intracavernosal pressure also decreased in SD group (b_image). CONCLUSIONS Acute SD may mainly exert influence on luteinizing hormone secretion in anterior hypophysis. Subsequently, the result decreases the steroidogenesis in leydig cell, resultantly decline of testosterone would develop. These chain-reactions of the hormone axis would be a factor of lowering the erectile function. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1113 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Dongsup Lee More articles by this author Seung-ju Lee More articles by this author Dong-wan Sohn More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...