MP58-05 URINARY SMART TEST FOR EARLY DETECTION OF PROSTATE CANCER

Abstract

You have accessJournal of UrologyCME1 May 2022MP58-05 URINARY SMART TEST FOR EARLY DETECTION OF PROSTATE CANCER Sergio Occhipinti, Daniele Amparore, Stefano Granato, Enrico Checcucci, Federico Piramide, Sabrina De Cillis, Alberto Piana, Gabriele Volpi, Paolo Verri, Michele Sica, Stefano Piscitello, Beatrice Carbonaro, Mariano Burgio, Juliette Meziere, Edoardo Cisero, Edoardo Dibilio, Giovanni Busacca, Matteo Manfredi, Cristian Fiori, and Francesco Porpiglia Sergio OcchipintiSergio Occhipinti More articles by this author , Daniele AmparoreDaniele Amparore More articles by this author , Stefano GranatoStefano Granato More articles by this author , Enrico CheccucciEnrico Checcucci More articles by this author , Federico PiramideFederico Piramide More articles by this author , Sabrina De CillisSabrina De Cillis More articles by this author , Alberto PianaAlberto Piana More articles by this author , Gabriele VolpiGabriele Volpi More articles by this author , Paolo VerriPaolo Verri More articles by this author , Michele SicaMichele Sica More articles by this author , Stefano PiscitelloStefano Piscitello More articles by this author , Beatrice CarbonaroBeatrice Carbonaro More articles by this author , Mariano BurgioMariano Burgio More articles by this author , Juliette MeziereJuliette Meziere More articles by this author , Edoardo CiseroEdoardo Cisero More articles by this author , Edoardo DibilioEdoardo Dibilio More articles by this author , Giovanni BusaccaGiovanni Busacca More articles by this author , Matteo ManfrediMatteo Manfredi More articles by this author , Cristian FioriCristian Fiori More articles by this author , and Francesco PorpigliaFrancesco Porpiglia More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002641.05AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Elevated PSA levels in blood tests are the gold standard for early prostate cancer detection, but the paradox is that it has long been known that advanced prostate cancers can loose PSA expression. We have observed that in the presence of tumors, the prostate looses the capacity to produce and secrete typical molecules, such as PSA and Zinc. The current study was designed to explore the possibility to develop a rapid urinary test to improve the detection of men at real risk of prostate cancer. METHODS: After prostatic massage urinary sample were collected, with informed consent, from 66 subjects that received indication for prostatic biopsies based on PSA levels, suspicious DRE examination or mpMRI evaluation. The amount of urinary PSA and Zinc was determined by an optimize lateral flow immunoassay and colorimetric dip-stick assay, respectively. RESULTS: Evaluation of biopsies showed the presence of localized neoplastic cells in 40 subjects (called hereafter CP), whereas remaining 26 subjects were free from disease (healthy subjects, HS). CP were stratified based on D’Amico criteria: 8 with low risk, 25 intermediate risk, 7 high risk prostatic tumors. First, we identified the optimal cutoff for both urinary PSA and Zinc the maximize the discrimination between CP and HS in term of sensitivity and specificity. Secondly, we design a Urine Score (uScore) scale based on the positivity of urinary biomarkers: 2 (both markers positive), 1 (only one marker positive), 0 (both markers negative). ROC curve analysis showed an higher capability in discriminating CP from HS of uScore compared to serum PSA (AUC 0.737 vs 0.524, p=0.029). Multivariate ROC curve analysis combining uScore with routine parameters such as PSA and Age and PiRADS obtained by mpMRI resulted in an AUC=0.894, that is statistically different from PSA+Age or PiRADS alone (p=0.03 and p=0.01, respectively). See Figure 1. CONCLUSIONS: These results suggest that urinary PSA and Zinc analysis could be clinically useful in detecting neoplastic transformation in the prostate and spare useless invasive analysis to healthy subjects. Rapid tests for urinary biomarkers could be an interesting approach to improve the diagnostic path of prostate cancer. Source of Funding: None © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e992 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sergio Occhipinti More articles by this author Daniele Amparore More articles by this author Stefano Granato More articles by this author Enrico Checcucci More articles by this author Federico Piramide More articles by this author Sabrina De Cillis More articles by this author Alberto Piana More articles by this author Gabriele Volpi More articles by this author Paolo Verri More articles by this author Michele Sica More articles by this author Stefano Piscitello More articles by this author Beatrice Carbonaro More articles by this author Mariano Burgio More articles by this author Juliette Meziere More articles by this author Edoardo Cisero More articles by this author Edoardo Dibilio More articles by this author Giovanni Busacca More articles by this author Matteo Manfredi More articles by this author Cristian Fiori More articles by this author Francesco Porpiglia More articles by this author Expand All Advertisement PDF DownloadLoading ...