MP57-20 EVALUATION OF GRAM POSITIVE AND GRAM NEGATIVE BACTERIA IN A NOVEL IN VITRO BIOFILM MODEL OF PENILE PROSTHESIS

Abstract

You have accessJournal of UrologySexual Function/Dysfunction: Surgical Therapy (MP57)1 Sep 2021MP57-20 EVALUATION OF GRAM POSITIVE AND GRAM NEGATIVE BACTERIA IN A NOVEL IN VITRO BIOFILM MODEL OF PENILE PROSTHESIS Manish Narasimman, Gregory Plano, Jesse Ory, Sara Schesser Bartra, and Ranjith Ramasamy Manish NarasimmanManish Narasimman More articles by this author , Gregory PlanoGregory Plano More articles by this author , Jesse OryJesse Ory More articles by this author , Sara Schesser BartraSara Schesser Bartra More articles by this author , and Ranjith RamasamyRanjith Ramasamy More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002087.20AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Infection is the most feared complication of inflatable penile prosthesis (IPP), often necessitating complete removal. Late infection after IPP insertion is thought to be due to the slow formation of a biofilm. Understanding and preventing biofilm formation would be a breakthrough in preventing IPP infections. We aimed to develop an in vitro model to compare formation and growth of biofilms on penile implants by bacteria commonly associated with infection. METHODS: Sterile IPPs (Coloplast® Titan) were cut into rings and incubated with S. epidermidis, S. aureus, P. aeruginosa, or K. pneumoniae cultures in tryptic soy broth (TSB) for a 4 h attachment period, and then in only TSB for 120 h. We utilized a published and validated method to measure biofilm mass. Rings were then dipped in deionized water to wash off additional bacteria and either fixed with ethanol (EtOH) or not treated further (no fix). Fixing was employed to preserve biofilm on the rings that otherwise may have been lost during crystal violet staining. Biofilm formation was measured with a spectrophotometer (OD570) after crystal violet staining. RESULTS: All bacteria formed substantial biofilm on implant rings as measured by OD570. As expected, the gram-negative bacteria, P. aeruginosa and K. pneumoniae produced significantly more biofilm than the gram-positive S. epidermidis and S. aureus. Interestingly, P. aeruginosa formed the most biofilm by at least a factor of 6 in both testing conditions. The ethanol fixing condition produced more consistent results for the crystal violet assay. CONCLUSIONS: Our novel in vitro model of biofilm formation of penile implants can be used in future studies to evaluate the effect of different antibiotic dips and anti-infective capacity of precoated implants against different bacteria. Our findings suggest gram-negative bacteria form faster and a greater quantity of biofilm on penile implants as compared to gram-positives. P. aeruginosa displayed the strongest predilection to form biofilm on implants compared to all other bacteria studied, and could be investigated as a specific target to reduce delayed post-operative IPP infections. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e987-e987 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Manish Narasimman More articles by this author Gregory Plano More articles by this author Jesse Ory More articles by this author Sara Schesser Bartra More articles by this author Ranjith Ramasamy More articles by this author Expand All Advertisement Loading ...