MP48-15 CIRCULAR RNA ITCH INHIBITS BLADDER CANCER PROGRESSION BY SPONGING MIR-17 AND REGULATING P21 EXPRESSION

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology II1 Apr 2017MP48-15 CIRCULAR RNA ITCH INHIBITS BLADDER CANCER PROGRESSION BY SPONGING MIR-17 AND REGULATING P21 EXPRESSION Pengchao Li, Jun Tao, Xiao Yang, Xiaheng Deng, and Qiang Lu Pengchao LiPengchao Li More articles by this author , Jun TaoJun Tao More articles by this author , Xiao YangXiao Yang More articles by this author , Xiaheng DengXiaheng Deng More articles by this author , and Qiang LuQiang Lu More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.1496AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Recently, Circular RNAs (CircRNAs) have emerged as critical regulators in tumorigenesis and progression. However, the role and molecular mechanism of CircRNA ITCH in bladder cancer is still unclear. METHODS Real-time PCR analysis was performed to measure the expression levels of circ-ITCH in bladder cancer tissues and cell lines. The biological function of circ-ITCH on bladder cancer cells were determined both in vitro and in vivo. Besides, bioinformatic databases including Starbase and CircInteractome were used to investigate the regulating relationship between circ-ITCH and miR-17 in bladder cancer cells. Western blotting was performed to detect the expression of p21 as a target of miR-17. Finally, the mechanism of competing endogenous RNA (ceRNA) between circ-ITCH and miR-17 was determined using bioinformatic analysis and luciferase assays. RESULTS Circ-ITCH was significantly down-regulated in bladder cancer tissues and cell lines. Overexpression of circ-ITCH exerted tumor-suppressive effects by inhibiting cell proliferation, migration and invasion in vitro and tumor growth in vivo. Furthermore, an inverse relationship between circ-ITCH and miR-17 was found. Circ-ITCH could partly abolish the effect that miR-17 targets p21 and lowers its expression consequently. CONCLUSIONS circ-ITCH is down-regulated significantly in bladder cancer, and the newly identified circ-ITCH/miR-17/p21 axis could be a potential biomarkers or therapeutic targets for bladder cancer patients. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e642-e643 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Pengchao Li More articles by this author Jun Tao More articles by this author Xiao Yang More articles by this author Xiaheng Deng More articles by this author Qiang Lu More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...