MP20-05 BLADDER INSTILLATION THEARPY OF MESENCYMAL STEM CELL AND THE ADDITION OF MANNOSE IN A RAT CYSTITIS MODEL- MULTIPLE SUPPRESSIVE EFFECTS FOR EXCESS CYTOKINES

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Kidney & Bladder II1 Apr 2015MP20-05 BLADDER INSTILLATION THEARPY OF MESENCYMAL STEM CELL AND THE ADDITION OF MANNOSE IN A RAT CYSTITIS MODEL- MULTIPLE SUPPRESSIVE EFFECTS FOR EXCESS CYTOKINES Tokunori Yamamoto, Yasuto Funahashi, Majima Tsuyoshi, Takai Syun, Yoshihisa Matsukawa, Hideki Mizuno, and Momokazu Gotoh Tokunori YamamotoTokunori Yamamoto More articles by this author , Yasuto FunahashiYasuto Funahashi More articles by this author , Majima TsuyoshiMajima Tsuyoshi More articles by this author , Takai SyunTakai Syun More articles by this author , Yoshihisa MatsukawaYoshihisa Matsukawa More articles by this author , Hideki MizunoHideki Mizuno More articles by this author , and Momokazu GotohMomokazu Gotoh More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.977AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Mesencymal stem cell(MSC) is used as a clinical regeneration therapeutic agent for various organs. We examined the healing effects of intravesical MSC on damaged urothelium in a rat model of chemically induced cystitis. METHODS Rat(r) and human(h)-MSC instillation in the bladder of female Sprague Dawley® and nude rats to cyclophosphamide induce cystitis respectively. After 8 hour r-MSC(n=6) ,r and h MSC with mannose(MN) (n=6),vehicle(n=6) were administered in the bladder for 48 hour. Histopathology, urothelial permeability, cystometrogram and nociceptive behaviors were evaluated on day 2. Bladder inflammation were evaluated the cytokaines of bladder urine and tissue measured by multipulex system and urine myeloperoxidase(MPO) measured by ELISA. RESULTS MN increased cell activity of MSC in vitro. Bladder histological evaluation revealed polymorphological inflammatory cell infiltration and increase in inflammatory and protect of damaged urothelium(Fig) and cytokines in urine and the tissue and MPO in urine. h-MSC and h-MSC with MN were homing in interstitialis of bladder of nude rats(Fig). Evans blue over absorption in the bladder wall were decreased in MSC and MSC with MN treated rats. These findings, which were associated with urothelial injury and increased permeability. Cystometrogram demonstrated that the intercontraction interval were shorter in MSC and MSC with MN treated rats furhthermore. CONCLUSIONS MSC and MSC with MN accelerated via multiple cytokines suppressive effects for excess cytokines the repair of damaged urothelium, protected urothelial barrier function and suppressed bladder overactivity and nociception respectively. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e224-e225 Peer Review Report Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tokunori Yamamoto More articles by this author Yasuto Funahashi More articles by this author Majima Tsuyoshi More articles by this author Takai Syun More articles by this author Yoshihisa Matsukawa More articles by this author Hideki Mizuno More articles by this author Momokazu Gotoh More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...