MP19-01 BLADDER FUNCTION IN A RAT MODEL OF BENIGN PROSTATIC HYPERPLASIA INDUCED WITH SEX HORMONES IN MALE WISTER RAT

Abstract

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research1 Apr 2014MP19-01 BLADDER FUNCTION IN A RAT MODEL OF BENIGN PROSTATIC HYPERPLASIA INDUCED WITH SEX HORMONES IN MALE WISTER RAT Seiji Matsumoto, Yuko Kawai, Michiko Oka, Tatsuya Oyama, Satoshi Okazaki, Kazumi Hashizume, Naoki Wada, Jun-Ichi Hori, Makoto Azumi, Masafumi Kita, Tatsuya Iwata, and Hidehiro Kakizaki Seiji MatsumotoSeiji Matsumoto More articles by this author , Yuko KawaiYuko Kawai More articles by this author , Michiko OkaMichiko Oka More articles by this author , Tatsuya OyamaTatsuya Oyama More articles by this author , Satoshi OkazakiSatoshi Okazaki More articles by this author , Kazumi HashizumeKazumi Hashizume More articles by this author , Naoki WadaNaoki Wada More articles by this author , Jun-Ichi HoriJun-Ichi Hori More articles by this author , Makoto AzumiMakoto Azumi More articles by this author , Masafumi KitaMasafumi Kita More articles by this author , Tatsuya IwataTatsuya Iwata More articles by this author , and Hidehiro KakizakiHidehiro Kakizaki More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.702AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We developed a rat model of benign prostatic hyperplasia (BPH) induced by sex hormones (daily injection of 0.25 mg/kg 17β-estradiol (E) add testosterone (T) in male Wister rat) and investigated the bladder function in this model. METHODS Ten-month-old male Wistar rats were divided into five groups: group 1 was treated with 0.1 ml sesame oil (sham; G1); group 2 was treated with E (G2); groups 3 - 5 were treated with E and T at 2.5 (E + 2.5T; G3), 12.5 (E + 12.5T; G4) and 25 (E + 25T; G5) mg/kg, respectively. On the 31st day after daily injection with E and T, we investigated several parameters; (1) voiding behavior in metabolic cages overnight for 12 hours, (2) bladder and prostate blood flow, (3) measurements of the bladder and prostate weight, (4) Bladder contractile responses to electrical field stimulation (EFS: 2, 8, 32 Hz), carbachol (100 μM), and KCl (100 mM). RESULTS Regarding voiding behavior, the number of micturitions was not significantly different among all groups. Average micturition volume in G4 significantly increased compared with G1. Although bladder blood flow was not significantly different among all groups, prostate blood flow in G5 tended to decrease compared with G1. Prostate weight significantly decreased in G2 and G3, but significantly increased in G5 compared with G1. Bladder weight significantly increased in G4 and G5 compared with G1. Bladder contractile forces in response to EFS, carbachol and KCl were not significantly different among all groups. CONCLUSIONS We previously reported that prostate weight decreased and bladder function was not affected in the E induced model in castrated Wistar rats [Int Urol Nephrol. 2013;45:749-54]. Tatemichi et al. reported that sex hormone treatment (only one dose with 0.125 mg/kg E and 12.5T) of young SD rats induced BPH and caused detrusor overactivity [J Urol. 2006;176:1236-41]. Rat BPH models differ in bladder function depending on the animal, age and dose of sex hormones. In our new rat BPH model induced by treatment with sex hormones, prostate and bladder weight increased and bladder function (detrusor contractile forces) was not affected. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e189 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Seiji Matsumoto More articles by this author Yuko Kawai More articles by this author Michiko Oka More articles by this author Tatsuya Oyama More articles by this author Satoshi Okazaki More articles by this author Kazumi Hashizume More articles by this author Naoki Wada More articles by this author Jun-Ichi Hori More articles by this author Makoto Azumi More articles by this author Masafumi Kita More articles by this author Tatsuya Iwata More articles by this author Hidehiro Kakizaki More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...