Mouse models advancing immunology research at The Jackson Laboratory

Abstract

Abstract The Jackson Laboratory (JAX) provides genetically-defined mice to the scientific community. This poster will highlight recently added novel models with immunology research application. The portfolio of strains useful for transplantation/PDX and “humanized” mouse research continues to expand and complement the NSG (NOD scid IL2rgc-/-) model as ideal tools for engraftment of human or mouse tissues. Novel fluorescent strains include Traffic Light Reporters for tracking DNA repair pathways, Gene Editing Reporters for detecting homology directed repair (HDR) and non-homologous end joining (NHEJ) repair events, as well as several CRISPR Editing, Bxb1 Integrase Editing, Base Editor and Prime Editor tools. Recently added CRISPR Array Repair Lineage Tracing (CARLIN and DARLIN) component strains allow researchers to achieve high barcode diversity in targeted cell populations. In addition to cryopreserving each strain, our genetic quality control program confirms mutation identity and genetic background, and screens for unwanted alleles (such as stray GFP, Cre, neo, etc.). Search the comprehensive collection using the improved JAX Mouse Search site (mice.jax.org). This resource includes models created by many generous donating institutions. Researchers are encouraged to donate their mouse lines via a very short Strain Submission form (jax.org/donate-a-mouse). JAX is supported by the NIH, The Howard Hughes Medical Institute and other private charitable foundations.