MOSSES DIVERSITY IN GROJOGAN SEWU, JUMOG, AND PARANG IJO WATERFALL, KARANGANYAR DISTRICT

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Moss is a plant that can be found in low and highlands and lives attached to the substrate. The purpose of this study was to determine the diversity of lichens that grow in waterfalls located in Karanganyar district. The method used in this research was purposive sampling method. Based on the observations of the species found came from 5 classes namely Bryopsida, Marchantiopsida, Anthecerotopsida, Hepaticopsida and Jungermanniopsida. The level of diversity in each waterfall is influenced by environmental factors such as temperature, humidity and sunlight. Jumog and Parang ijo waterfalls have a high diversity index, while the evenness value of the three waters is high, and has the same dominance value which is classified as low.

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Intratumoral genetic heterogeneity leads to tumor progression and therapeutic resistance. However, due to the difficulty associated with its assessment, application of intratumoral genetic heterogeneity as a prognostic or predictive marker is limited. We investigated the significance of Shannon diversity index as a tool for measuring genetic heterogeneity by performing fluorescence in situ hybridization of c-Myc gene in invasive breast cancers, and correlated the Shannon diversity index for c-Myc copy number variation with clinicopathologic features of tumor including patient survival. Shannon index for c-Myc copy number variation strongly correlated with average c-Myc copy number and was significantly higher in tumors with c-Myc genetic or regional heterogeneity than in those without c-Myc amplification. High Shannon index was associated with high histologic grade, lymphovascular invasion, p53 overexpression, high Ki-67 proliferation index, hormone receptor negativity, and HER2 amplification. In survival analyses, c-Myc amplification or c-Myc copy number gain was not associated with patient survival. However, a high level of Shannon index was associated with poor disease-free survival. In subgroup analyses, it was found to be an adverse prognostic factor in hormone receptor-positive group, but not in hormone receptor-negative group. In a validation set, high Shannon diversity index for c-Myc copy number variation was also found to be associated with poor survival of the patients. We further investigated the correlation with clinicopathologic features and predictive power of Shannon index using another gene, fibroblast growth factor receptor 1 (FGFR1) and observed that high Shannon index for FGFR1 gene copy number variation was an independent prognostic factor for poor clinical outcome in a whole group and in hormone receptor-positive subgroup. In conclusion, this study demonstrated that higher diversity index was associated with adverse pathologic parameters of breast cancer and poor clinical outcome suggesting that Shannon diversity index, which represents intratumoral genetic heterogeneity, can be used as a potential biomarker for tumor progression and prognostication in patients with breast cancer. Citation Format: Yul Ri Chung, Hyun Jeong Kim, Young A Kim, Mee Soo Chang, Ki-Tae Hwang, So Yeon Park. Diversity index as a novel predictor of tumor progression in breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3937. doi:10.1158/1538-7445.AM2017-3937

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Abstract. Ghazali M, Widoretno W, Arumingtyas EL, Retnaningdyah C. 2022. Macroepiphyte biodiversity on Kappaphycus alvarezii surface and its interaction with environment in cultivation centers on Lombok Island, Indonesia. Biodiversitas 23: 6284-6292. The aims of this paper are to investigate the influence of environmental factors on the diversity of macroepiphytes in Kappaphycus alvarezii. Macroepiphyte sampling was carried out at four cultivation centers: Seriwe Bay, Ekas Bay, Gerupuk Bay, and Siwak of Lombok Island, West Nusa Tenggara, Indonesia. Several stations were placed at each location, and at each station were used one to three cultivation units with five replications. Environmental data measurements were carried out at the time of sampling. Some parameters were measured: pH, temperature, salinity, current velocity, brightness, DO, BOD, nitrate, phosphate, and TSS. Data analysis was performed using several diversity indices. In addition, a biplot analysis was performed using PCA to analyze the correlation between environmental factors and environmental factors to macroepiphytes. The results showed that the most dominant species was Polysiphonia spaerocharpa, and the second was Ceramium sp1. The highest richness and abundance were found in Ekas Bay. The highest species dominance was in Siwak. The highest diversity index was in Ekas Bay. The correlation between environmental factors showed that hemeroby harmed depth, phosphate, and DO and positively affected BOD and the thallus surface structure. The principal correlation analysis showed that the abundance of macroepiphyte was influenced by BOD, the roughness of host thallus, and the hemeroby index.

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