Abstract
Adult mosquitoes were collected for virus isolation from two sites in Indian River County, FL, from May 5 through August 13, 1998 using dry ice-baited CDC light traps (81 trap-nights) and CDC gravid traps (254 trap-nights). A total of 46,150 female mosquitoes (923 mosquito-pools, 50 females/pool) were processed for virus isolation. These females represented 18 species of mosquitoes, with Culex nigripalpus comprising 77.4% of all mosquitoes collected, followed by Aedes infirmatus (4.9%), Ae. vexans (4.0%) and Cx. erraticus (2.4%). No St. Louis encephalitis (SLE) and eastern equine encephalitis (EEE) virus isolates were obtained. Keystone (KEY) and Tensaw (TEN) viruses were isolated from Ae. albopictus (one isolate of KEY); Anopheles crucians (two isolates of TEN); Cx. nigripalpus (one isolate of TEN and 2 isolates of KEY); Coquilletidia perturbans (two isolates of TEN); and Wyeomyia vanduzeei (one isolate of TEN). All isolates were obtained from mosquitoes collected in CDC light traps, except for the KEY virus isolate from Ae. albopictus, which was collected in a CDC gravid trap. The isolation of TEN virus from Wy. vanduzeei is a first record for Florida.
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