Morphology and dendritic domains of neurons in the lateral parabrachial nucleus of the rat.

Abstract

The present study provides a description of the dendritic morphology and the dendritic domains of neurons in the lateral parabrachial nucleus (PB) of the rat. The cells were intracellularly stained in vitro with Lucifer yellow. A subpopulation of these cells was characterized beforehand as neurons projecting to the amygdaloid complex by retrograde transport with rhodamine beads. With respect to their dendritic arborization, different types of "spatially" organized PB neurons were discriminated. One major cell type in the external lateral PB (PBel) is characterized by long, elongated dendritic trees that are preferentially oriented parallel to the superior cerebellar peduncle. The majority of their dendrites appears to respect subnuclear boundaries, yet their distal dendrites often exceed the limits of the PBel to encroach upon adjacent subnuclei located dorsally and ventrolaterally to the PBel. Another prominent cell type in the PBel has fairly small and locally restricted dendritic trees that are also elongated, running with their main axis from ventrolateral to dorsomedial. The dendrites of the majority of these neurons apparently stay within the confines of the PBel. A distinct group of neurons is found in the ventral portion of the PBel. The majority of their dendrites is mediolaterally oriented and not confined to the PBel subnucleus. In addition, we found a smaller number of neurons scattered within the lateral PB whose dendrites do not show a preferential orientation but travel across subnuclear boundaries into several different PB subnuclei. Our data show that the dendrites of a large proportion of neurons in the lateral PB either stay within the confines of a particular subnucleus or slightly extend across subnuclear limits. In any case, they appear to match with terminal territories of afferent axons and, thus, maintain the functional specificity of inputs by their relay through the PB. In contrast, PB neurons that extend their dendrites across subnuclear boundaries or known terminal territories are likely to receive inputs of different qualities from a variety of sources and therefore transmit a more general, integrated signal to the forebrain.