Morphological Features of Wound Regeneration in the Local Application of Recombinant Interferon Α2b in the Experiment

Abstract

Summery. Introduction. The research devoted to the study of pathogenetic, immunological, and biophysical aspects of the tissue regeneration process with wound defects of various etiologies is gaining in popularity in the "world" of medical science, at present this problem remains topical [1, 2, 6, 8, 9]
 Purpose of the study. A comparative analysis of the dynamics of morphological manifestations of tissue regeneration occurring when recombinant interferon α2b, is used in the healing of an acute experimental wound is carried out.
 Materials and methods. Depending on the nature of the wound treatment, the experimental animals were divided into two groups: 1) a comparison group in which the edges of the wound were struck with a 0.9% NaCl solution; 2) the main group, where a similar edge of the wound defect was cut off with recombinant interferon α2b at a dose of 1000 ME / 1ml diluted with 0.9% NaCl solution. The rats were removed from the experiment on the 4th, 8th and 20th day of the study, while a full-thickness skin flap was taken in the area of the scar (defect), which was examined by light microscopy. At the same time, the intensity of formation of the granulation tissue, its "maturation", and also in the margins and on the bottom of the wound was estimated in the absolute values, the cellular populations realizing the process of inflammation were determined.
 As a result of the study, the character of the complex effect of recombinant interferon α2b was established, which included reduction of the negative effect of secondary alteration, differentiation of the intravascular immune response, formation of conditions for natural regeneration with activation of angiogenesis and synthesis of a valuable scar, decrease in propensity to keloidosis.
 Conclusions. The obtained results testify to the morphological validity of the use of recombinant leukocyte interferon α2β as a stimulant of the inflammation phase, as against the background of the use of this substance, its ability to enhance phagocytosis was noted.