Abstract
The work is a fragment of the research project “Pathogenetic features of the allergic and inflammatory processes course and their pharmacocorrection”, conducted by the Department of Pathological Physiology, Danylo Halytskyi Lviv National Medical University (state registration No. 0116U004503).
 The aim of the work: to study the morphological features of the colon in the dynamics of the development of experimental acute peritonitis on the background of streptozotocin-induced diabetes mellitus.
 Materials and Methods. The experiment used 48 white male rats. Diabetes mellitus in experimental animals was simulated by a single intraperitoneal injection of streptozotocin (Sigma) at a rate of 60 mg/kg, acute disseminated peritonitis – the introduction of 0.5 ml of 10 % filtered fecal suspension into the abdominal cavity. A morphological study of the colon in animals removed from the experiment on the first, third and seventh days of acute peritonitis on the background of concomitant diabetes mellitus was performed.
 Results and Discussion. Morphological examination of the colon of animals on the first day of experimental acute peritonitis in conditions of concomitant streptozotocin-induced diabetes mellitus revealed an increase in the size of the crypts due to stroma edema and lymphohistiocytic infiltration, slight perivascular edema in the subclavian edema. On the third day, thickening of the mucous membrane of the colon, a sharp increase in the depth of the crypts, uneven blood supply to the vessels in the submucosal layer with a predominance of perivascular edema were verified. On the seventh day, a decrease in the thickness of the mucous membrane due to the expansion of the crypts was visualized. Part of the epitheliocytes was in a phase of increased secretory activity, the other part was dystrophically altered, which stimulated increased lymphocytic and histiocytic infiltration.
 These changes were accompanied by activation of reactive processes and hyperplasia of lymphoid follicles on the first day of peritonitis on the background of streptozotocin-induced diabetes mellitus, and as the severity of purulent inflammation – hypoplasia of the lymphoid tissue and a decrease in local reactivity(the third and seventh days of the development of acute peritoneal burning in conditions of combined pathology).
Highlights
Despite the introduction of the new methods of diagnosis and treatment, wide range of antimicrobial drugs, the mortality of patients with acute peritonitis remains high at 15.5 % to 37.8 % [1, 2, 3]
Increased vascular permeability and edema as a result of hyperglycemia lead to the development of multiple organ failure [8], which is the cause of death in patients with acute generalized peritonitis (AGP) [9, 10]
On the se venth day of acute peritonitis against the background of diabetes, dystrophic changes of the epithelium and the absence of lymphoid follicles in the submucosa were verified, which indicates a hypoergic course of this combined pathology
Summary
Despite the introduction of the new methods of diagnosis and treatment, wide range of antimicrobial drugs, the mortality of patients with acute peritonitis remains high at 15.5 % to 37.8 % [1, 2, 3]. Increased vascular permeability and edema as a result of hyperglycemia lead to the development of multiple organ failure [8], which is the cause of death in patients with acute generalized peritonitis (AGP) [9, 10]. One of the pathogenetic links of multiple organ failure is the enteral insufficiency syndrome, characterized by the increasing intestinal paresis, sequestration of fluid and gases, accompanied by pronounced morphological changes in the wall of the small and large intestine, which are the gateway to bacterial contamination. In the scientific literature there is no data about the morphological structure of the large intestine in the hypoergic course of the inflammatory process of the peritoneum under the influence of hyperglycemia, which was the trigger for our study. The aim of the work was to study the morphological features of the large intestine in the dynamics of experimental AGP against the background of streptozotocin (STZ)-induced diabetes
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