Abstract
Some aspects influencing the achievement of transgenic bean (Phaseolus vulgaris L.) plants have been studied. The morphology of the shoot apex in 17 cultivars within the Carioca and Jalo types was analyzed. In some cultivars the apical meristematic region was partially covered by the primordia leaves. Cultivars in which the meristematic area was 100% exposed were assessed for multiple shoot induction using three concentrations of benzylaminopurine (4.4 FiM, 22.2 FLM, and 44.4 FLM). The cultivar PF9029984 showed prolific multiple shoot induction. The early events of multiple shoot formation were examined using scanning electron microscopy. Transgenic bean plants from a Carioca-type cultivar were obtained by particle bombardment. The inheritance of the introduced foreign gene was Mendelian in all transgenic bean lines. Southern blot hybridization confirmed the integration of foreign genes into the plant genome.
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