Abstract

Objective:
 To investigate the effect of nicotine on the morphology of an inflamed reconstituted oral mucosa in vitro when treated for 24 hours.
 Materials and Methods:
 This observational laboratory-based study focuses on the effects of nicotine on an in vitro reconstituted model oral mucosa. The reconstituted human epithelium model used in the study was prepared and supplied by Skin Ethic Laboratories, Nice, France. The effect of the different treatments of nicotine on tissue morphology was assessed using formalin-fixed paraffin wax sections and haematoxylin and eosin staining.
 Results:
 It was found that the effect of nicotine after 24 hours with working solutions (10μM and 10mM) used on inflamed oral mucosal did not significantly affect on gross morphology.
 Conclusion:
 The concentration of nicotine used after 24 hours did not affect tissue morphology.

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