Abstract
Azotobacter chroococcum B-8 was nutritionally stressed for iron (Fe) and molybdenum (Mo) by successive transfers on Fe-deficient and Mo-deficient solid-plating media and was subsequently observed with transmission electron microscopy after capsular staining in ruthenium red. Under Fe- and Mo-sufficient conditions, the capsule appeared to be condensed into a thin, electron-dense layer. Under iron stress, and to a lesser extent molybdenum stress, the capsule appeared more diffuse and extensive. This apparent diffusion was irreversible upon the addition of iron before processing. Thus there seems to be a correlation between capsule diffusion and metal starvation, as well as a correlation between capsule extent and metal starvation.
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