Abstract
Morphological Comparison in Spermatozoa from Italian and Egyptian Buffalo Bulls, and their IVF Outcome
Highlights
Italian frozen semen gained high popularity in the Egyptian market for the enhancement of farm animal productivity, and genetic improvement
Oocytes maturation in vitro: Oocyte maturation step was performed according to the report by Mahmoud et al(2016a) with modification, 10-20 oocytes were cultured in 100 μl small drop of TCM-199 added with 10% fetal calf serum (FCS), 50 μg/ml gentamycin sulfate and 50 μM cysteamine covered with mineral oil
Frozen straws were thawed at 37oCfor30 sin a water bath, and washed by centrifugation (800×g/ 10 min) in bovine serum albumin (BSA)free Brackett and Oliphant (BO) medium with 10 μg/ ml heparin and 2.5 mM caffeine
Summary
Italian frozen semen gained high popularity in the Egyptian market for the enhancement of farm animal productivity (especially milk yield), and genetic improvement. The fertility of Italian frozen-thawed semen is better than that of the Egyptian breed and the reason for that variation is still elusive. Variations among bulls in artificial insemination (AI) centers concerning the non-return rates variations as much as 25%, and these differences cannot be detected using the routine semen analysis (Larson and Miller, 2000). The differences in metabolic characteristics of the sperm cell (Brackett and Oliphant, 1975), the ratio of sperm number to seminal plasma volume (Fukui et al, 1988), seminal plasma content and, plasma membrane composition of sperm (Alvarez and Storey, 1995) are employed to explain the variation in pregnancy or in vitro fertilization (IVF) rates between sires
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