Abstract

Abstract. Inyod I, SassanarakitS, PayapanonA, Keawsompong S. 2017. Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa). Biodiversitas 18: 221-228. Hed Taen Rad or Macrocybe crassa is a wild edible mushroom found in Thailand. It has a large fruiting body with a meaty texture and a delicious taste. Five strains of Hed Taen Rad; DOA, DOA-1, DOA-4, DOA-7 and DOA-10 were tested in different media, pH values and temperatures to measure mycelium growth kinetics and identify mycelia and basidiomata. Results indicated that mushroom mycelial growth rates were highest on MEA medium at pH values between 8 and 10, and temperatures between 20°C and 30°C. Identification of the isolates was based on the morphology characteristics of the basidiomata were closely similar to M. crassa (Berk.), and different from M. gigantea (Massee). For molecular analysis by sequencing of their internal transcribed spacer (ITS) regions was identified as a T. giganteum since limited ITS rDNA sequences from M. crassa exist in public databases. Therefore, the identification of five strains of Hed Taen Rad using molecular methods could be erroneous. Therefore, morphology characteristics study is suitable for further analysis method on genetic study of Hed Taen Rad.
 Keywords: Hed Taen Rad, Macrocybe crassa, molecular analysis, morphological characteristics

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.