MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS IN PIG BLOOD UNDER USE OF LG-MAX AND SEL-PLEX FEED ADDITIVES IN FEEDING

The acquired domestic and foreign experience of pig breeding development, an increase of animal productivity , and reduction of pork cost by 65–70% and more is determined by scientifically substantiated feeding. At the same time, the biochemical parameters of pig blood serum can be used to assess the functional state of the body and predict animal productivity and meat quality. The purpose of the study was to determine the morphological and biochemical blood parameters in young pigs with the inclusion of LG-Max and Sel-Plex feed additives to the main diet. When using in pig feed ing LG-MAX feed additive at a dose of 4.0 g/day, the blood biochemical parameters in pigs of the experimental groups compared with the control were within physiological limits according to the age of the animals. At the same time, during the experimental period it was found that the biochemical parameters in blood serum of pigs of the experimental group compared to the control of the appropriate age are in the following dynamics: in 120 - day -old pigs , significantly increases the content of total protein, the concentration of γ-globulins and aspartate aminotransferase , decreases the content of albumin, glucose, creatinine, cholesterol , and alkaline phosphatase; in 155-day-old pigs, the concentration of γ-globulins and the albumin/globulin ratio significantly increases and the urea, calcium , and chloride content increases; cholesterol and iron levels were significantly lower. Keywords: biochemical parameters, blood serum, LG-MAX feed additive

The research objective was to determine haematological and biochemical parameters in blood of 30 Lacaune dairy sheep during different stages of lactation (early: 60th, medium: 120th and late: 180th days). The sheep were on average 4 years old, in 3rd lactation. Haematological parameters were determined in whole blood (white blood cell (WBC), red blood cell (RBC), haemoglobin (HGB) concentration, haematocrit (HCT) value, mean corpuscular volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and platelet (PLT) count), and a blood smear was used for determination of differential blood cell count. Determined biochemical parameters in blood serum were: concentration of minerals (Ca, P-inorganic, Mg and Fe), concentration of total proteins (TP), albumin (ALB), globulins (GLOB), urea, glucose (GUK), cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TGC), beta-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFA), enzyme activities (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyl transferase (GGT), creatine kinase (CK), activity of glutathione peroxidase (GPx) and total superoxide dismutase (SOD). With progression of lactation stages, sheep blood tests proved significant increase in WBC, RBC, HGB, HCT, monocytes, Fe, urea, TP, ALB, CHOL, HDL, LDL, BHB, AST, GGT and ALT as well as a decrease in lymphocytes, Mg, GUK and SOD. Determined changes in haematological and biochemical blood parameters of Lacaune dairy sheep during different lactation stages proved good quality nutrition of sheep and indicate the need to include this dairy breed/genotype in prospective schemes for definition of referential values for these indicators in sheep.

The scientific article presents the results of research on the development of an effective method for isolating nucleic acids by citrate-salt hydrolysis from the freeze-dried biomass of Chlorella vulgaris microalgae to create an oral preparation based on them in the form of a sodium salt of nucleic acids, as well as determining its effect on morphological and biochemical parameters of rabbit blood, in particular protein metabolism. A special bioreactor was developed and constructed for the experiment. The author's method of extracting nucleic acids from the biomass of green microalgae of the genus Chlorella vulgaris was developed and tested to obtain the final product in the form of sodium nucleinate powder with the necessary physico-chemical parameters, which was determined by appropriate analysis methods. The yield of sodium nucleinate after drying in an air current and weighing on an electronic scale with an error of ± 0.0002 g in mass ratios was 6.0 g from a sample of 50.0 g of dry biomass of Chlorella vulgaris microalgae. The resulting preparation is a white - beige finely dispersed amorphous powder, odorless after the stages of washing and drying. Electrophoretic analysis determined that the preparation of nucleic acids from chlorella has a molecular weight of 350 to 400 kDa. It has been established that the obtained preparation based on nucleic acids has a positive effect on morphological and biochemical parameters of blood and indicators of protein metabolism of rabbits. The increase in the content of total protein, albumins, gamma globulins in rabbits receiving the drug was more pronounced compared to the control group of rabbits. An increase in the level of gamma globulins in rabbits treated with a nucleic preparation indicates the activation of humoral protection factors in them.

In this study, (24) Awassi rams were used at the age of (1.5-2 years), with an average weight of (46.4 kg) to study the effect of giving some antioxidants on hematological and blood biochemical on Awassi rams , The rams were randomly allotted into four groups of equal number (6) rams each group , the first group (control) , the second group was given turmeric at the rate of 3 g / kg feed / head daily, the third group was supplemented orally with 400 IU vitamin E / head three times a week , the fourth group was supplemented orally with 400 mg CoQ10 coenzyme / head three times a week , and these materials were given to the rams of each treatment before providing the daily feed to the animals , The experimental animals were given a standardized diet consisting of ( concentrated feed + Wheat Straw ) on the basis of 2.5 % of live body weight . The percentage of protein in the diet was 15.22% and the metabolic energy was (2721.5) kcal / kg of feed. The weights of the animals were measured and blood samples were drawn from the jugular vein every month to measure the characteristics of the physical and biochemical parameters of the blood in the blood serum. The results of the study showed a significant (p?0.01) effect of the treatments on the characteristics of the animal’s weight, number of WBC, the percentage of eosinophil's and basophils than control group , while the treatments did not show a significant effect on the other blood values. The results also showed a significant effect of the treatments on the level of concentration of total protein, cholesterol, triglycerides, globulin and urea, in addition to the levels of HDL, LDL , While there was no significant effect of the treatments on the concentration of glucose, albumin, VLDL in the blood plasma. Through the results of this study, we conclude that giving antioxidants to rams had a positive effect on some physical, biochemical and enzymatic parameters of blood in the treated groups compared to the control.

It has been established that the blood test is one of the quick methods of assessing the physiological state of the body under the action of exogenous stimuli, what is important when testing new feed additives in pig feeding. Studies of morphological blood parameters showed that feeding young pigs with starter feed with feed additive “Activo” helped to increase the red blood cells and hemoglobin level. Thus, the hemoglobin content in the blood of pigs of the second group increased by 7.5 % compared to the control. The difference between the control and experimental groups for red blood cells was highly significant (P < 0.001) in favor of the latter. The content of leukocytes and eosinophils in the blood of young animals of the experimental group increased in comparison with the same parameter of control group, while the content of bacillary and segmented granulocytes and monocytes is insignificantly reduced or at the control level. Biochemical parameters of blood showed that in experimental animals the content of total protein increased by 19.4 %, albumin by 11.1 %, globulins by 3.07 %. These results were obtained as a result of scientific and economic experiment on two groups-analogues of young large white breed pigs of PIC genetics, 25 animals each. The initial live weight of piglets was 12 kg. The starting feed of piglets consisted of barley stubble – 28 %, wheat – 29.79 %, corn – 15 %, soybean oilcake – 22.91 %, premix “Nutrimin” – 4.3 %. During the main period of the experiment, the animals of the experimental group received the feed additive “Activo” in the amount of 0.2 kg t, which was produced by the German company EW Nutrition GmBH. The level of feeding provided average daily gains by groups within the following limits: 562 and 621 g respectively control and experimental groups. The average daily weight gain of young pigs of the second group was higher than the control group by 52 g, or 9.14 % (P ˂ 0.001). Accordingly, the absolute gain in these animals increases by 1.4 kg. Feed conversion rate per 1 kg of gain decreased by 0.18, or 8.5 % (P < 0.001) comparing to the first.

It has been established that the blood test is one of the quick methods of assessing the physiological state of the body under the action of exogenous stimuli, what is important when testing new feed additives in pig feeding. Studies of morphological blood parameters showed that feeding young pigs with starter feed with feed additive “Activo” helped to increase the red blood cells and hemoglobin level. Thus, the hemoglobin content in the blood of pigs of the second group increased by 7.5 % compared to the control. The difference between the control and experimental groups for red blood cells was highly significant (P < 0.001) in favor of the latter. The content of leukocytes and eosinophils in the blood of young animals of the experimental group increased in comparison with the same parameter of control group, while the content of bacillary and segmented granulocytes and monocytes is insignificantly reduced or at the control level. Biochemical parameters of blood showed that in experimental animals the content of total protein increased by 19.4 %, albumin by 11.1 %, globulins by 3.07 %. These results were obtained as a result of scientific and economic experiment on two groups-analogues of young large white breed pigs of PIC genetics, 25 animals each. The initial live weight of piglets was 12 kg. The starting feed of piglets consisted of barley stubble – 28 %, wheat – 29.79 %, corn – 15 %, soybean oilcake – 22.91 %, premix “Nutrimin” – 4.3 %. During the main period of the experiment, the animals of the experimental group received the feed additive “Activo” in the amount of 0.2 kg t, which was produced by the German company EW Nutrition GmBH. The level of feeding provided average daily gains by groups within the following limits: 562 and 621 g respectively control and experimental groups. The average daily weight gain of young pigs of the second group was higher than the control group by 52 g, or 9.14 % (P ˂ 0.001). Accordingly, the absolute gain in these animals increases by 1.4 kg. Feed conversion rate per 1 kg of gain decreased by 0.18, or 8.5 % (P < 0.001) comparing to the first.

The use of probiotics in animal feed is one of the most promising alternatives to the application of antibiotics. In order to establish the effectiveness of the use of a complex probiotic supplement (Lactobacillus spp., Lactococcus spp., Spirulinaceae, Saccharomyces cerevisiae, Actinomycetaceae, Aspergillus spp. and Penicillium spp.) in pig farming, two groups of pigs of the Poltava Meat breed were formed by the analog method. In the ration of the fattening pigs of the experimental group, 1.0% of the mass of compound feed was replaced with a complex probiotic supplement, which was fed during the 45 days of the experimental period at the age of 135 to 180 days. In experimental pigs, biochemical blood parameters were determined (the content of protein, lipids, cholesterol, triglycerides, activity of aspartate aminotransferase and alanine aminotransferase, the content of creatinine, glucose, calcium, phosphorus, albumins and globulins) using test kits from the company "Filisit Diagnostics" (UA). As a result of the research, it was established that with the use of a complex probiotic feed supplement, the content of total protein in the blood serum of the pigs of the experimental group was higher by 18.4%, glucose by 21.4%, aspartate aminotransferase activity by 20.4%, alanine aminotransferase by 38.0% compared to control group. In both groups, regardless of the established difference, the named indicators were within the physiological norm. The concentration of cholesterol in the blood of pigs receiving the probiotic supplement decreased by 43.2%, but the concentration of calcium (by 26.2%) and phosphorus (by 17.9%) increased. In the experimental group, the blood indicators related to the immunity of pigs improved – the content of α1 and α2 globulins decreased by 4.1% and 7.4%, respectively. Also, in the experimental group, the concentration of albumins increased by 7.5%, which probably indicates an improvement in the functional state of the liver. As a result of feeding with a complex probiotic supplement in the experimental group the albumin-globulin ratio improved, which increased by 35.0%. Thus, the introduction of a complex probiotic feed additive into the ration of pigs changed the structure of the influence of exogenous and endogenous factors on metabolism, had a positive effect on the protein profile of the blood, and caused an increase of average daily gains in experimental animals by 5.8% compared to the control group.

This study was conducted to ascertain the influence of addition thymol powder to the diet on growth performance, nutrient digestibility and some blood biochemical parameters of lambs fed a diet consisting of 60% concentrated and 40% alfalfa hay. Sixteen Arabi male lambs were randomly distributed into four groups; the first group lambs were received diet without any addition and served as control group, while the second, third, and fourth groups lambs were received 200, 300 and 400 mg thymol.kg-1 dry matter, respectively, for 105 days. The results showed that the supplement groups had a significantly higher (P≤0.05) in total and daily weight gain, feed conversion ratio, and feed intake than in control group. Lambs received 300 mg thymol.kg-1 significantly improved (P≤0.05) digestibility coefficient of dry matter and nitrogen free extract as compared with control lambs. Lambs received 300 mg thymol.kg-1 feed) were higher (P>0.05) than those received 200 mg thymol.kg-1 feed in terms of organic matter, crude fiber, cellulose, and hemicellulose digestibility coefficient. Blood urea concentration was significantly lower (P≤0.05) in the second group (200 mg thymol.kg-1) than control group. While other blood parameters such as glucose, total protein, albumin, globulin, cholesterol, triglycerides, phosphorus, and calcium concentrations were not affected by thymol addition. It can be concluded that addition thymol at 300 mg.kg-1 enhanced growth performance and nutrient digestibility in Arabi lambs without adverse effect on blood biochemical parameters.

Currently it has become popular to use different feed additives that allow us to increase the indicators of live weight gain by accelerating the metabolic processes in the body of animals, which allows us to take everything from the feed and to the maximum. With the advent of this trend in the market the range of feed additives in different directions has increased. For the research we have selected Vermiculite Expanded as a feed additive. The purpose of the research was to search the dynamics of changes in blood parameters in experimental steers in comparison with the control group against the background of the use of the feed additive Vermiculite Expanded. An assessment of blood parameters of Kazakh White-headed steers against the background of the use of mineral feed additive has been presented in the article. Three groups of steers have been formed for the research: the 1st group has been as a control and received the main economic ration; the 2nd and 3rd experimental groups received the feed additive to the main feeding ration in doses of 10 and 15 g/head for the groups, respectively. On the laboratory base blood tests we were able to search the correlation between morphological and biochemical parameters of blood and the growth rate of experimental steers. The steers of the 2nd and 3rd groups during the experiment had an advantage in blood serum indicators in terms of both total lipids and glucose, in the end, the difference in percentage ratio with the control group was 14,08 % in the 2nd group and 12,60 % in the 3rd experimental group. It is worth noting that the concentration of phosphorus and calcium in the blood was at the high level, which in our opinion reflects the influence of the feed additive Vermiculite Expanded.

Due to their durability, versatility and economy, plastic products are widely used in all spheres of human life. Despite the inertness of polymers, recent studies show the ability of microplastic to overcome natural tissue barriers, accumulate in the animal’s body, affect metabolism and change the intestinal microbiota, negatively affecting it. In a 42-day experiment, changes in the internal organs’ relative mass, blood biochemical and morphological parameters of white mice were established under the influence of different doses of polystyrene foam in their diet. Four groups of white mice consumed crushed polystyrene foam particles (10%, 1% and 0.1% by weight of the feed, control group without the addition of polystyrene foam). At the end of the experiment, the morphofunctional state of the internal organs was determined by the organ mass index and blood biochemical parameters. Adding crushed polystyrene foam to the feed in an amount of 1% causes a significant decrease in the mass index of the heart and stomach, 10% – only the heart, and 0.1% – does not affect this indicator. Polystyrene foam had a significant effect on blood biochemical parameters, regardless of the dose, causing an increase in the activity of aspartate aminotransferase against the background of a decrease in the activity of alkaline phosphatase. The content of total bilirubin, urea, urea nitrogen and cholesterol decreased, and the concentration of creatinine and total protein increased (due to the albumin fraction). The use of crushed polystyrene foam in mice did not cause significant changes in the blood morphological composition, except for a dose-dependent increase in the number of monocytes. In the future, it is planned to determine histological, histochemical and immunohistochemical changes in the organs of laboratory animals under the influence of plastic in a laboratory experiment.

Cattle thelaziosises are helminthic diseases caused by the parasitization of the Spirurata nematodes of the Thelaziidae family. The course of the disease is characterized by the development of conjunctivitis, keratoconjunctivitis and corneal ulcer, which, in the absence of adequate treatment leads to loss of vision in animals and their culling. The complex drugs are shown high therapeutic efficacy in the fight against this invasion. One of them is ophthalmectin - a safe and highly effective drug based on ivermectin and azithromycin. The aim of the study was to study the acceptability (safety) of ophthalmectin for target animal species. The experiment was carried out on 24 healthy calves of red-and-white breed belonging to "Agrosvet" CHSC of Kashirskiy district of Voronezh region. The investigated drug was once injected into the cavity of the conjunctival sac from 0.5 g (therapeutic dose) to 2.0 g. The drug was not injected to the calves of the control group. The toxic effect of ophthalmectin was assessed by clinical condition of animals, morphological and biochemical parameters of blood. Throughout the study pulse rate, numbers of respiratory movements, number of cardial impulse, rumen contraction were within the limits of physiological norm for this type of animals. Single use of the drug in the investigated doses did not lead to changes in morphological and biochemical parameters of calf blood. As a result of the study of ophthalmectin acceptability (safety) in target animals it was found out that injection of the drug once in the cavity of the conjunctival sac of the eye of calves in the therapeutic dose of 0.5 g and four times more does not affect to the condition of animals and is well tolerated by them.

Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch)

Currently, along with an in-depth study of the gene pool and the phenotype pool of farm animals of different breeds and species in the zones of their breeding, special attention is paid to the production of environmentally friendly and safe products of the agro-industrial complex for the end consumer. Therefore, water, feed, soil, animal organs and tissues are carefully monitored for the content of chemical elements. Manganese, like magnesium and other divalent ions, is a non-specific activator of enzymes: hydrolases, kinases, decarboxylases, etc. The absolute need for Mn is confirmed by the metalloenzymes pyruvate carboxylase of the liver mitochondria and muscle oxaloacetate carboxylase. The first contains four manganese atoms and four biotin molecules and catalyzes the carboxylation of pyruvic acid to oxaloacetic acid. The removal of Mn leads to the irreversible loss of enzyme activity, which is not restored with the subsequent introduction of the endogenous metal. The purpose of the research was to establish the correlations of the accumulation of manganese in the hairs with some biochemical parameters in the blood of pigs of Kemerovskaya breed. During the work the correlations of manganese in the hairs with some biochemical parameters of blood serum of pigs have been studied. When comparing the obtained data with the studies of other scientists, all the correlations were described except for the content of manganese in the hairs with serum chlorides (r=–0,43), since this correlation was not confirmed by the data of the scientific literature. A positive correlation has been established between the level of Mn with cholesterol and Mg at the level of 0,542 and 0,417, respectively. The change in the magnitude and direction of the correlation between the concentration of Mn in the hairs and the biochemical composition of the blood can be caused by some animal diseases.

본 연구는 꽃사슴에게 고농도의 셀레늄을 급여하였을 때 꽃사슴의 혈액, 녹용 및 생리반응에 미치는 영향을 평가하고자 실시하였다. 3두의 꽃사슴에게 고농도 셀레늄을 급여하였을 때의 혈액 내의 셀레늄 농도와 셀레늄 흡수율 및 축적률을 조사하고, 혈액 내 스트레스 관련호르몬 및 biochemical parameters를 조사하였다. 8두의 수컷 꽃사슴을 공시하여 셀레늄을 첨가하지 않은 사료를 급여한 구와 셀레늄을 사료 kg당 6mg을 첨가한 사료를 20일간 급여한 구로 나누어 생산한 녹용의 셀레늄 함량을 조사하였다. 꽃사슴에게 고농도 셀레늄 급여는 급여 30일 이후에 혈액 중의 셀레늄 농도를 유의하게 증가시켰고(p<0.05), 축적률은 59.15%를 나타내었다.전 실험 기간 동안 고농도 셀레늄의 급여로 인한 스트레스 관련 호르몬 및 biochemical parameters의 변화는 나타나지 않았다. 녹용 내 셀레늄 함량은 대조구의 경우 상대 0.11ppm, 중대 0.08ppm, 하대에 0.08ppm을 셀레늄 급여구에서는 상대 0.45ppm(p<0.001), 중대 0.21ppm(p<0.01), 하대에 0.15ppm(p<0.05)으로 셀레늄 급여로 인하여 유의하게 증가하였으며, 상대에서의 셀레늄 함량이 가장 높았다(p< 0.05).이상의 결과로부터 꽃사슴에게 고농도의 셀레늄을 급여할 경우 녹용내로 다량의 셀레늄을 전이시킬 수 있는 것으로 판단되었고, 40일까지 고농도의 셀레늄 6mg/kg 농도 급여에 있어 생리적 중독 증상은 없었으나 혈중 소실정도를 감안해 볼 때 20일간 급여하는 것이 적합하다고 사료된다. 향후 셀레늄 강화 녹용 생산을 위해 급여하는 셀레늄의 최적 농도와 급여 기간에 대한 연구가 더 필요할 것으로 사료된다. This study was conducted to investigate the effects of selenium feeding and supplementation in diet on the concentration of selenium in blood and velvet antler of spotted deer(Sika deer). Three spotted deer were fed high selenium concentration(6mg/kg DM). Absorption and retention rates of selenium were examined by evaluating selenium concentrations in feces and urine. Stress-related hormones and serum biochemical parameters in blood were also evaluated for the purpose of detecting any negative effect by the high level of selenium feeding. Eight spotted deers were randomly assigned to two groups and were fed with one of two diets for 20 days, which were with or without the addition of 6mg selenium /kg diet. Concentration of selenium in velvet antler was evaluated. Selenium concentration in blood of spotted deer fed high level selenium for 30 days was significantly increased(p<0.05), retention rate of selenium reached 59.15%. No differences in level of stress-related hormone and biochemical parameters(NEFA, ALT, AST) in blood were observed by feeding high level selenium. The diet with selenium significantly increased concentrations of selenium in top(0.11 vs 0.45ppm; p<0.001), middle(0.08 vs 0.21ppm; p<0.01) and basepart(0.08 vs 0.15ppm; p<0.05) of velvet antler.

This study was conducted in the field of the Poultry Research Station of the Department of Animal Production / Department of Agricultural Research / Ministry of Agriculture for the period 4/4/2021 to 16/5/2021, in which 300 one-day-old Ross308 chicks that fed on diets used avocado oil and Chia with percentages 0, 0.2, 0.4, 0.6% respectively, and their mixture consisting of 0.0, 0.1, 0.2, 0.3 each of avocado and Chia oil (50% avocado + 50% Chia oil). The experiment included 4 treatments with 3 replicates for each treatment (10 birds/replicates), in order to study the effect of using avocado and chia oil and their mixture in meat broiler diets on some physiological and microbial characteristics of blood plasma. The results indicate a significant increase (P &lt; 0.01) in the concentration of total protein and globulin for the treatment birds in which the mixture of avocado and Chia oil was used. While there were no significant differences in the concentration of albumin between the types of the used oils. It was noted through the results that the percentage of usage 0.6% had achieved a high significant increase in the concentration of total protein, albumin and globulin in the blood plasma of the birds of these treatments compared with the rest of the percentages of use (0, 0.2 and 0.4). As for the interaction, the results showed a high significant increase in the protein concentration (P&lt;0.01) in the treatment (M0.6) in both total protein and albumin concentration compared with the rest of the interactions, And the concentration of globulin was significantly increased (P&lt;0.01) in the birds of the treatments (C0.6, M0.2, M0.4, M0.6) compared with the rest of the interactions. The results showed a high significant decrease (P&lt;0.01) in the concentration of cholesterol in the birds of the treatments in which the mixture of oil and Chia oil were used, while a high significant increase (P &lt; 0.01) in the concentration of HDL was observed in the birds that used the mixture of avocado and Chia oil in their diets compared with the other types of oils, While a high significant decrease (P&lt;0.01) in LDL concentration was observed in birds of the treatment with the oil mixture, while no significant differences were observed between types of oils in the concentration of both triglycerides and VLDL. The level of the percentage of usage had a high significant decrease (P &lt; 0.01) in the concentration of cholesterol, triglycerides, LDL and VLDL at all levels of usage 0.2, 0.4 and 0.6% compared to the control treatment, while a high significant (P &lt; 0.01) increase in the concentration of HDL was observed at the rates of use of 0.4 and 0.6% compared with the control treatment which is void of usage. Whereas, the best high significant improvement (P&lt;0.01) in the lipid profile of the lipid plasma was achieved by a mixture treatment with a percentage of use of 0.6% compared with the rest of the interactions. It was noted that there was a high significant increase (P &lt; 0.01) in the number of Lactobacillus bacteria and a high significant decrease (P &lt; 0.01) in the number of E. coli bacteria in the avocado and Chia oil mixture treatments compared with the rest of the used oils. As for the study of the effect of the percentage of use, it increased significantly. (P &lt; 0.01) Lactobacillus bacteria numbers of all usage percentages (0.2, 0.4 and 0.6) compared with the control treatment, in addition to a high significant decrease (P &lt; 0.01) in the numbers of E. coli bacteria. As for the interaction between the type of oil and the percentage of usage, a high significant increase (P &lt; 0.01) was observed in the numbers of Lactobacillus bacteria in the treatment (M0.2 and M0.6) compared with the rest of the interactions, in addition to a high significant decrease (P &lt; 0.01) In the numbers of E.coli bacteria in treatment (M0.6) as the best interaction compared to the rest of the interactions. We conclude from this study that the use of mixed oil at a percentage of 0.4-0.6% in meat broiler diets will improve the physiological characteristics of the blood plasma, in addition to a decrease in the number of harmful bacteria (E. coli) and an increase in the number of beneficial bacteria, which has an important role in improving the physiological condition of birds, which is reflected positively on productive performance and obtaining high growth without the use of harmful synthetic materials.