Abstract
In this paper, a nano-porous polymer has been integrated into the microfluidics device as on-chip monolithic liquid chromatography column for separation of chemical and biological samples. Monolithic nano-porous polymer (MNP) was formed and firmly grafted on the surface of the microfluidic channel. Neurotransmitters, 5-hydroxyindole-3-acetic acid (5-HIAA) and 5-hydroxytryptamine (serotonin, 5-HT), were successfully separated with the developed on-chip MNP column.
Highlights
In recent years, microfabricated systems have been considered as promising tools for chemical and biological analysis because of the rapid analysis and control of minute samples [1,2,3,4,5,6,7,8]
Monolithic stationary phases potentially offer the advantages of the simple control of permeability and surface areas and easy preparation for application within a micro-fluidic channel for lab-on-a chip (LOC) [14–17]
In terms of the grafted Polyethylene terephthalate (PET) samples, the contact angle began to decrease from 5 min UV exposure and the lowest contact angle was measured on the sample formed by 10 min UV exposure
Summary
In recent years, microfabricated systems have been considered as promising tools for chemical and biological analysis because of the rapid analysis and control of minute samples [1,2,3,4,5,6,7,8]. Since separation of chemical components is an important technique in bioanalysis, various trials have been conducted to integrate separation functionality onto the micro-total analysis system (μ-TAS) [9,10,11]. Liquid chromatography (LC) is the most popular separation technique [12, 13]. Monolithic stationary phases potentially offer the advantages of the simple control of permeability and surface areas and easy preparation for application within a micro-fluidic channel for lab-on-a chip (LOC) [14–17]. LC separation columns were formed in TPE channels using nano-porous polymer as a monolithic column. Permeability was calculated from back-pressures measured as a function of flow-rates. Neurotransmitters were separated by the developed on-chip monolithic LC column to validate separation performance
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