Abstract
Introduction: Oxidative stress represents a pathophysiological mechanism lying behind occurrence of different acute and chronic diseases. Pregnancy, mainly due to placenta rich with mitochondria, is also being associated with the state of oxidative stress. Numerous markers have been proposed in order to test oxidative stress in pregnancy state, one of them being 8-isoprostane. The aim of this study was to analyse serum concentrations of 8-isoprostane as a possible oxidative stress marker in pregnancy.Methods: Serum concentrations of 8-isoprostane were measured in overall population of 84 woman, between 20 and 30 years of age. Tested population was divided in 2 groups: 42 pregnant woman were classifi ed as being either in the fi rst or second trimester of pregnancy. In the control group healthy nonpregnant women were included. Concentration of 8-isoprostane in serum was determined by commercial 8-isoprostane EIA test kit of Cayman Chemical Company, USA.Results: The 8-isoprostane levels were signifi cantly increased in pregnant women in relation to healthy non pregnant women (p<0.05). The 8-isoprostane levels were signifi cantly increased in second and third trimester of pregnancy (p<0.05).Conclusions: According to the obtained results, 8-isoprostane might be used as a possible marker of oxidative stress in pregnancy state, but not as a biomarker for the risk of complications development in pregnancy in analysed population.
Highlights
Oxidative stress represents a pathophysiological mechanism lying behind occurrence of different acute and chronic diseases
Numerous studies suggest that 8-isoprostane as an oxidative stress indicator is increased in pregnancy and in pathological states of pregnancy [11,12,13,14,15,16,17,18,19,20], while a few suggest no differences in its levels during development of pregnancy [4]
This seems to be in accordance with results obtained in our study where higher level of 8- isoprostane was detected in both, first and second trimester pregnant woman when compared to controls, proving out the fact that pregnancy by itself is a state of oxidative stress [7,8]
Summary
Oxidative stress represents a pathophysiological mechanism lying behind occurrence of different acute and chronic diseases. Oxidative stress generally describes a state where a cell antioxidative defence is inadequate to com-. The main consequence of oxidative stress is the damage of DNA, lipids and proteins, which compromise the health of a cell or induce production of different reactive compounds leading to the death of the cells by necrosis or apoptosis. Lipid peroxidation can lead to increased cell damage through piling of oxidative products, out of which some are chemically reactive and modify macromolecules by creating covalent bounds. Different and relatively weaker final products are created by lipid peroxidation, mostly α,β- unsaturated reactive aldehydes, such as malondialdehyde (MDA), 4-hidroxynonenal (HNE), 2-propenal (acrolein) and isoprostanes, which can be determined in plasma and urine and serve as indirect oxidative stress indicators [2]
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